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[KO Validated] MAP2K2 Rabbit pAb (A14770)

Datasheet

ABclonal:Western blot - [KO Validated] MAP2K2 Rabbit pAb (A14770)
ABclonal:Western blot - [KO Validated] MAP2K2 Rabbit pAb (A14770)
ABclonal:Immunofluorescence - [KO Validated] MAP2K2 Rabbit pAb (A14770)
ABclonal:Immunofluorescence - [KO Validated] MAP2K2 Rabbit pAb (A14770)

Overview

Product name[KO Validated] MAP2K2 Rabbit pAb
Catalog No.A14770
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is known to play a critical role in mitogen growth factor signal transduction. It phosphorylates and thus activates MAPK1/ERK2 and MAPK2/ERK3. The activation of this kinase itself is dependent on the Ser/Thr phosphorylation by MAP kinase kinase kinases. Mutations in this gene cause cardiofaciocutaneous syndrome (CFC syndrome), a disease characterized by heart defects, mental retardation, and distinctive facial features similar to those found in Noonan syndrome. The inhibition or degradation of this kinase is also found to be involved in the pathogenesis of Yersinia and anthrax. A pseudogene, which is located on chromosome 7, has been identified for this gene.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-100 of human MAP2K2 (NP_109587.1).
SequenceMLARRKPVLPALTINPTIAEGPSPTSEGASEANLVDLQKKLEELELDEQQKKRLEAFLTQKAKVGELKDDDFERISELGAGNGGVVTKVQHRPSGLIMAR
Gene ID5605
Swiss protP36507
SynonymsCFC4; MAPKK2; MEK2; MKK2; PRKMK2; MAP2K2
Calculated MW44kDa
Observed MW45KDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
IHC 1:100 - 1:200
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesHeLa, 293T, HT-29
Cellular locationCytoplasm, Membrane, Peripheral membrane protein
ABclonal:Western blot - [KO Validated] MAP2K2 Rabbit pAb (A14770) }

Western blot - [KO Validated] MAP2K2 Rabbit pAb (A14770)

Western blot analysis of extracts of HeLa cells, using MAP2K2 antibody (A14770) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Western blot - [KO Validated] MAP2K2 Rabbit pAb (A14770) }

Western blot - [KO Validated] MAP2K2 Rabbit pAb (A14770)

Western blot analysis of extracts of various cell lines, using MAP2K2 antibody (A14770) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunofluorescence - [KO Validated] MAP2K2 Rabbit pAb (A14770) }

Immunofluorescence - [KO Validated] MAP2K2 Rabbit pAb (A14770)

Immunofluorescence analysis of NIH/3T3 cells using MAP2K2 antibody (A14770) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - [KO Validated] MAP2K2 Rabbit pAb (A14770) }

Immunofluorescence - [KO Validated] MAP2K2 Rabbit pAb (A14770)

Immunofluorescence analysis of U2OS cells using MAP2K2 antibody (A14770) at dilution of 1:100. Blue: DAPI for nuclear staining.

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