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[KO Validated] MAP2K1 Polyclonal Antibody (A12687)

Datasheet

ABclonal:Western blot - [KO Validated] MAP2K1 Polyclonal Antibody (A12687)
ABclonal:Western blot - [KO Validated] MAP2K1 Polyclonal Antibody (A12687)

Overview

Product name[KO Validated] MAP2K1 Polyclonal Antibody
Catalog No.A12687
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-100 of human MAP2K1 (NP_002746.1).
SequenceMPKKKPTPIQLNPAPDGSAVNGTSSAETNLEALQKKLEELELDEQQRKRLEAFLTQKQKVGELKDDDFEKISELGAGNGGVVFKVSHKPSGLVMARKLIH
Gene ID5604
Swiss protQ02750
SynonymsMAP2K1; CFC3; MAPKK1; MEK1; MKK1; PRKMK1; mitogen-activated protein kinase kinase 1
Calculated MW40kDa/43kDa
Observed MW45kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide Array
Recommended dilutionWB 1:1000 - 1:3000
IHC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    
Positive samplesJurkat, HeLa, A-431, MCF-7, Mouse Liver, Mouse Kidney, Rat Thymus
Cellular locationCytoplasm, Membrane, Nucleus, Peripheral membrane protein, centrosome, cytoskeleton, microtubule organizing center, spindle pole body
ABclonal:Western blot - [KO Validated] MAP2K1 Polyclonal Antibody (A12687) }

Western blot - [KO Validated] MAP2K1 Polyclonal Antibody (A12687)

Western blot analysis of extracts of various cell lines, using MAP2K1 antibody (A12687) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.
ABclonal:Western blot - [KO Validated] MAP2K1 Polyclonal Antibody (A12687) }

Western blot - [KO Validated] MAP2K1 Polyclonal Antibody (A12687)

Western blot analysis of extracts from normal (control) and MAP2K1 knockout (KO) HeLa cells, using MAP2K1 antibody (A12687) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

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