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[KO Validated] Lamin A/C Rabbit mAb (A19524)

Tested applications:WB IHC ICC IF IP ChIP ChIP-seq RIP FC ELISA MeDIP Nucleotide Array DB FACS CoIPReactivity:Human, Mouse, Rat

ABclonal:Western blot - [KO Validated] Lamin A/C Rabbit mAb (A19524)

Western blot analysis of extracts from wild type (WT) and Lamin A/C knockout (KO) HeLa cells, using Lamin A/C antibody (A19524) at 1:50000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

Western blot analysis of extracts of various cell lines, using [KO Validated] Lamin A/C Rabbit mAb antibody (A19524) at 1:500000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.

Western blot analysis of extracts from wild type (WT) and Lamin A/C knockout (KO) HeLa cells, using Lamin A/C antibody (A19524) at 1:500000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.

ABclonal:Immunohistochemistry - [KO Validated] Lamin A/C Rabbit mAb (A19524)

Immunohistochemistry of paraffin-embedded Human breast cancer using Lamin A/C antibody (A19524) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Immunohistochemistry of paraffin-embedded human esophageal cancer using Lamin A/C antibody (A19524) at dilution of 1:400 - 1:3200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - [KO Validated] Lamin A/C Rabbit mAb (A19524)

Immunofluorescence analysis of H9C2 cells using Lamin A/C antibody (A19524) at dilution of 1:200. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of L929 cells using Lamin A/C antibody (A19524) at dilution of 1:200. Blue: DAPI for nuclear staining.

Confocal immunofluorescence analysis of HeLa cells using Lamin A/C antibody (A19524) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of U2OS cells using Lamin A/C antibody (A19524) at dilution of 1:100 - 1:1600. Blue: DAPI for nuclear staining.

ABclonal:Immunoprecipitation - [KO Validated] Lamin A/C Rabbit mAb (A19524)

Immunoprecipitation analysis of 300ug extracts of PC-12 cells using 3ug Lamin A/C antibody (A19524). Western blot was performed from the immunoprecipitate using Lamin A/C antibody (A19524) at a dilition of 1:500000.

Review

Overview

Product name	[KO Validated] Lamin A/C Rabbit mAb
Catalog No.	A19524
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. Alternative splicing results in multiple transcript variants. Mutations in this gene lead to several diseases: Emery-Dreifuss muscular dystrophy, familial partial lipodystrophy, limb girdle muscular dystrophy, dilated cardiomyopathy, Charcot-Marie-Tooth disease, and Hutchinson-Gilford progeria syndrome.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 403-572 of human Lamin A/C (NP_733821.1).
Sequence	SSHSSQTQGGGSVTKKRKLESTESRSSFSQHARTSGRVAVEEVDEEGKFVRLRNKSNEDQSMGNWQIKRQNGDDPLLTYRFPPKFTLKAGQVVTIWAAGAGATHSPPTDLVWKAQNTWGCGNSLRTALINSTGEEVAMRKLVRSVTVVEDDEDEDGDDLLHHHHGSHCSS
Gene ID	4000
Swiss prot	P02545
Synonyms	LMNA; CDCD1; CDDC; CMD1A; CMT2B1; EMD2; FPL; FPLD; FPLD2; HGPS; IDC; LDP1; LFP; LGMD1B; LMN1; LMNC; LMNL1; MADA; PRO1; lamin; Lamin A/C
Calculated MW	62-74kDa
Observed MW	68KDa/72KDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC ICC IF IP ChIP ChIP-seq RIP FC ELISA MeDIP Nucleotide Array DB FACS CoIP
Recommended dilution	WB 1:50000 - 1:200000 IHC 1:50 - 1:1000 IF 1:50 - 1:1000 IP 1:50 - 1:200
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Application key	Western blotting Immunohistochemistry Immunofluorescence Immunoprecipitation
Positive samples	HeLa, A-549, NIH/3T3, PC-12
Cellular location	Nucleus, Nucleus envelope, Nucleus lamina, Nucleus speckle, nucleoplasm
Customer validation	(Caenorhabditis Elegans) WB(Mus musculus)