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[KO Validated] Lamin A/C Rabbit mAb (A19524)

Review (1)Publications (2) Datasheet

ABclonal:Immunohistochemistry - [KO Validated] Lamin A/C Rabbit mAb (A19524)

ABclonal:Immunofluorescence - [KO Validated] Lamin A/C Rabbit mAb (A19524)

ABclonal:Immunoprecipitation - [KO Validated] Lamin A/C Rabbit mAb (A19524)

Review

Overview

Product name	[KO Validated] Lamin A/C Rabbit mAb
Catalog No.	A19524
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. Alternative splicing results in multiple transcript variants. Mutations in this gene lead to several diseases: Emery-Dreifuss muscular dystrophy, familial partial lipodystrophy, limb girdle muscular dystrophy, dilated cardiomyopathy, Charcot-Marie-Tooth disease, and Hutchinson-Gilford progeria syndrome.

Immunogen information

Immunogen	Recombinant protein of human Lamin A/C.
Sequence	Email for sequence
Gene ID	4000
Swiss prot	P02545
Synonyms	LMNA; CDCD1; CDDC; CMD1A; CMT2B1; EMD2; FPL; FPLD; FPLD2; HGPS; IDC; LDP1; LFP; LGMD1B; LMN1; LMNC; LMNL1; MADA; PRO1; lamin; Lamin A/C
Calculated MW	62-74kDa
Observed MW	62kDa/74kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC ICC IF IP ChIP ChIPseq RIP FC ELISA MeDIP Nucleotide Array
Recommended dilution	WB 1:50000 - 1:200000 IHC 1:50 - 1:1000 IF 1:50 - 1:1000 IP 1:50 - 1:200
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key	Western blotting Immunohistochemistry Immunofluorescence Immunoprecipitation
Positive samples	K-562, A-549, NIH/3T3, Rat lung, HeLa, HeLa whole cell protein, HeLa cytoplasm protein, HeLa nuclear protein, NIH/3T3 whole cell protein, NIH/3T3 cytoplasm protein, NIH/3T3 nuclear protein, C6 whole cell protein, C6 cytoplasm protein, C6 nuclear protein
Cellular location	Nucleus, Nucleus envelope, Nucleus lamina, Nucleus speckle, nucleoplasm
Customer validation	(Caenorhabditis Elegans) IF(Homo sapiens) WB(Homo sapiens,Mus musculus) IF(Homo sapiens,Mus musculus)

Research Area

[KO Validated] Lamin A/C Rabbit mAb images

Western blot - [KO Validated] Lamin A/C Rabbit mAb (A19524)

Western blot analysis of extracts of HeLa cells, using Lamin A/C antibody (A19524) at 1:10000-1:2560000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

Western blot - [KO Validated] Lamin A/C Rabbit mAb (A19524)

Western blot analysis of extracts of various cell lines, using Lamin A/C antibody (A19524) at 1:50000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

Western blot - [KO Validated] Lamin A/C Rabbit mAb (A19524)

Western blot analysis of extracts of various cell lines, using Lamin A/C antibody (A19524) at 1:10000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

Western blot - [KO Validated] Lamin A/C Rabbit mAb (A19524)

Western blot analysis of extracts from normal (control) and Lamin A/C knockout (KO) HeLa cells, using Lamin A/C antibody (A19524) at 1:50000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

Immunohistochemistry - [KO Validated] Lamin A/C Rabbit mAb (A19524)

Immunohistochemistry of paraffin-embedded Human breast cancer using Lamin A/C antibody (A19524) at dilution of 1:200 (40x lens).

Immunohistochemistry - [KO Validated] Lamin A/C Rabbit mAb (A19524)

Immunohistochemistry of paraffin-embedded human esophageal cancer using Lamin A/C antibody (A19524) at dilution of 1:400 - 1:3200 (40x lens).