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[KO Validated] Lamin A/C Rabbit mAb (A19524)

Review (1) Datasheet

ABclonal:Western blot - [KO Validated] Lamin A/C Rabbit mAb (A19524)
ABclonal:Western blot - [KO Validated] Lamin A/C Rabbit mAb (A19524)
ABclonal:Western blot - [KO Validated] Lamin A/C Rabbit mAb (A19524)
ABclonal:Western blot - [KO Validated] Lamin A/C Rabbit mAb (A19524)
ABclonal:Immunohistochemistry - [KO Validated] Lamin A/C Rabbit mAb (A19524)
ABclonal:Immunohistochemistry - [KO Validated] Lamin A/C Rabbit mAb (A19524)
ABclonal:Immunofluorescence - [KO Validated] Lamin A/C Rabbit mAb (A19524)
ABclonal:Immunofluorescence - [KO Validated] Lamin A/C Rabbit mAb (A19524)
ABclonal:Immunofluorescence - [KO Validated] Lamin A/C Rabbit mAb (A19524)
ABclonal:Immunofluorescence - [KO Validated] Lamin A/C Rabbit mAb (A19524)
ABclonal:Immunoprecipitation - [KO Validated] Lamin A/C Rabbit mAb (A19524)
Review
ABclonal: review for [KO Validated] Lamin A/C Rabbit mAb(A19524)

Overview

Product name[KO Validated] Lamin A/C Rabbit mAb
Catalog No.A19524
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. Alternative splicing results in multiple transcript variants. Mutations in this gene lead to several diseases: Emery-Dreifuss muscular dystrophy, familial partial lipodystrophy, limb girdle muscular dystrophy, dilated cardiomyopathy, Charcot-Marie-Tooth disease, and Hutchinson-Gilford progeria syndrome.
ImmunogenRecombinant protein of human Lamin A/C.
SequenceEmail for sequence
Gene ID4000
Swiss protP02545
SynonymsLMNA; CDCD1; CDDC; CMD1A; CMT2B1; EMD2; FPL; FPLD; FPLD2; HGPS; IDC; LDP1; LFP; LGMD1B; LMN1; LMNC; LMNL1; MADA; PRO1; lamin; Lamin A/C
Calculated MW62-74kDa
Observed MW62kDa/74kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:50000 - 1:200000
IHC 1:50 - 1:1000
IF 1:50 - 1:1000
IP 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 1% BSA, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive samplesK-562, A-549, NIH/3T3, Rat lung, HeLa, HeLa whole cell protein, HeLa cytoplasm protein, HeLa nuclear protein, NIH/3T3 whole cell protein, NIH/3T3 cytoplasm protein, NIH/3T3 nuclear protein, C6 whole cell protein, C6 cytoplasm protein, C6 nuclear protein
Cellular locationNucleus, Nucleus envelope, Nucleus lamina, Nucleus speckle, nucleoplasm
Customer validation

(Caenorhabditis Elegans)

ABclonal:Western blot - [KO Validated] Lamin A/C Rabbit mAb (A19524) }

Western blot - [KO Validated] Lamin A/C Rabbit mAb (A19524)

Western blot analysis of extracts of HeLa cells, using Lamin A/C antibody (A19524) at 1:10000-1:2560000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Western blot - [KO Validated] Lamin A/C Rabbit mAb (A19524) }

Western blot - [KO Validated] Lamin A/C Rabbit mAb (A19524)

Western blot analysis of extracts of various cell lines, using Lamin A/C antibody (A19524) at 1:50000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Western blot - [KO Validated] Lamin A/C Rabbit mAb (A19524) }

Western blot - [KO Validated] Lamin A/C Rabbit mAb (A19524)

Western blot analysis of extracts of various cell lines, using Lamin A/C antibody (A19524) at 1:10000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Western blot - [KO Validated] Lamin A/C Rabbit mAb (A19524) }

Western blot - [KO Validated] Lamin A/C Rabbit mAb (A19524)

Western blot analysis of extracts from normal (control) and Lamin A/C knockout (KO) HeLa cells, using Lamin A/C antibody (A19524) at 1:50000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Immunohistochemistry - [KO Validated] Lamin A/C Rabbit mAb (A19524) }

Immunohistochemistry - [KO Validated] Lamin A/C Rabbit mAb (A19524)

Immunohistochemistry of paraffin-embedded Human breast cancer using Lamin A/C antibody (A19524) at dilution of 1:200 (40x lens).
ABclonal:Immunohistochemistry - [KO Validated] Lamin A/C Rabbit mAb (A19524) }

Immunohistochemistry - [KO Validated] Lamin A/C Rabbit mAb (A19524)

Immunohistochemistry of paraffin-embedded human esophageal cancer using Lamin A/C antibody (A19524) at dilution of 1:400 - 1:3200 (40x lens).
ABclonal:Immunofluorescence - [KO Validated] Lamin A/C Rabbit mAb (A19524) }

Immunofluorescence - [KO Validated] Lamin A/C Rabbit mAb (A19524)

Immunofluorescence analysis of H9C2 cells using Lamin A/C antibody (A19524) at dilution of 1:200. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - [KO Validated] Lamin A/C Rabbit mAb (A19524) }

Immunofluorescence - [KO Validated] Lamin A/C Rabbit mAb (A19524)

Immunofluorescence analysis of L929 cells using Lamin A/C antibody (A19524) at dilution of 1:200. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - [KO Validated] Lamin A/C Rabbit mAb (A19524) }

Immunofluorescence - [KO Validated] Lamin A/C Rabbit mAb (A19524)

Confocal immunofluorescence analysis of HeLa cells using Lamin A/C antibody (A19524) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - [KO Validated] Lamin A/C Rabbit mAb (A19524) }

Immunofluorescence - [KO Validated] Lamin A/C Rabbit mAb (A19524)

Immunofluorescence analysis of U2OS cells using Lamin A/C antibody (A19524) at dilution of 1:100 - 1:1600. Blue: DAPI for nuclear staining.
ABclonal:Immunoprecipitation - [KO Validated] Lamin A/C Rabbit mAb (A19524) }

Immunoprecipitation - [KO Validated] Lamin A/C Rabbit mAb (A19524)

Immunoprecipitation analysis of 200ug extracts of K-562 cells using 3ug Lamin A/C antibody (A19524). Western blot was performed from the immunoprecipitate using Lamin A/C antibody (A19524) at a dilition of 1:20000.

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