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[KO Validated] LGALS3 Rabbit pAb (A13506)

Review (1) Datasheet

ABclonal:Western blot - [KO Validated] LGALS3 Rabbit pAb (A13506)
ABclonal:Western blot - [KO Validated] LGALS3 Rabbit pAb (A13506)
ABclonal:Immunofluorescence - [KO Validated] LGALS3 Rabbit pAb (A13506)
ABclonal:Immunofluorescence - [KO Validated] LGALS3 Rabbit pAb (A13506)
ABclonal:Immunofluorescence - [KO Validated] LGALS3 Rabbit pAb (A13506)
Review
ABclonal: review for [KO Validated] LGALS3 Rabbit pAb(A13506)

Overview

Product name[KO Validated] LGALS3 Rabbit pAb
Catalog No.A13506
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the galectin family of carbohydrate binding proteins. Members of this protein family have an affinity for beta-galactosides. The encoded protein is characterized by an N-terminal proline-rich tandem repeat domain and a single C-terminal carbohydrate recognition domain. This protein can self-associate through the N-terminal domain allowing it to bind to multivalent saccharide ligands. This protein localizes to the extracellular matrix, the cytoplasm and the nucleus. This protein plays a role in numerous cellular functions including apoptosis, innate immunity, cell adhesion and T-cell regulation. The protein exhibits antimicrobial activity against bacteria and fungi. Alternate splicing results in multiple transcript variants.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-1-250 of human LGALS3 (NP_002297.2).
SequenceMADNFSLHDALSGSGNPNPQGWPGAWGNQPAGAGGYPGASYPGAYPGQAPPGAYPGQAPPGAYPGAPGAYPGAPAPGVYPGPPSGPGAYPSSGQPSATGAYPATGPYGAPAGPLIVPYNLPLPGGVVPRMLITILGTVKPNANRIALDFQRGNDVAFHFNPRFNENNRRVIVCNTKLDNNWGREERQSVFPFESGKPFKIQVLVEPDHFKVAVNDAHLLQYNHRVKKLNEISKLGISGDIDLTSASYTMI
Gene ID3958
Swiss protP17931
SynonymsLGALS3; CBP35; GAL3; GALBP; GALIG; L31; LGALS2; MAC2
Calculated MW26kDa
Observed MW31kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunofluorescence    
Positive samplesHT-29, SGC-7901, HeLa, Mouse brain, Mouse kidney
Cellular locationCytoplasm, Nucleus, Secreted
Customer validation

(mouse cells)

ABclonal:Western blot - [KO Validated] LGALS3 Rabbit pAb (A13506) }

Western blot - [KO Validated] LGALS3 Rabbit pAb (A13506)

Western blot analysis of extracts of various cell lines, using LGALS3 antibody (A13506) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Western blot - [KO Validated] LGALS3 Rabbit pAb (A13506) }

Western blot - [KO Validated] LGALS3 Rabbit pAb (A13506)

Western blot analysis of extracts from normal (control) and LGALS3 knockout (KO) HeLa cells, using LGALS3 antibody (A13506) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Immunofluorescence - [KO Validated] LGALS3 Rabbit pAb (A13506) }

Immunofluorescence - [KO Validated] LGALS3 Rabbit pAb (A13506)

Immunofluorescence analysis of C6 cells using LGALS3 antibody (A13506) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - [KO Validated] LGALS3 Rabbit pAb (A13506) }

Immunofluorescence - [KO Validated] LGALS3 Rabbit pAb (A13506)

Immunofluorescence analysis of L-929 cells using LGALS3 antibody (A13506) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - [KO Validated] LGALS3 Rabbit pAb (A13506) }

Immunofluorescence - [KO Validated] LGALS3 Rabbit pAb (A13506)

Immunofluorescence analysis of U-2 OS cells using LGALS3 antibody (A13506) at dilution of 1:100. Blue: DAPI for nuclear staining.

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