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[KO Validated] LC3B Rabbit pAb (A11282)

Publications (20) Datasheet

Tested applications:WBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIPReactivity:Human, Mouse, Rat

ABclonal:Western blot - [KO Validated] LC3B Rabbit pAb (A11282)

Western blot analysis of extracts of various cell lines, using LC3B antibody (A11282) at 1:500 dilution.NIH/3T3 cells were treated by Chloroquine (50 μM) at 37℃ for 20 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Western blot - [KO Validated] LC3B Rabbit pAb (A11282)

Western blot analysis of extracts of 293T cells, using LC3B antibody (A11282) at 1:500 dilution.293T cells were treated by Chloroquine (50 μM) at 37℃ for 20 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunohistochemistry - [KO Validated] LC3B Rabbit pAb (A11282)

Immunohistochemistry of paraffin-embedded mouse brain using [KO Validated] LC3B Rabbit pAb (A11282) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - [KO Validated] LC3B Rabbit pAb (A11282)

Immunohistochemistry of paraffin-embedded rat brain using [KO Validated] LC3B Rabbit pAb (A11282) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Overview

Product name[KO Validated] LC3B Rabbit pAb
Catalog No.A11282
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-125 of human LC3B (NP_073729.1).
SequenceMPSEKTFKQRRTFEQRVEDVRLIREQHPTKIPVIIERYKGEKQLPVLDKTKFLVPDHVNMSELIKIIRRRLQLNANQAFFLLVNGHSMVSVSTPISEVYESEKDEDGFLYMVYASQETFGMKLSV
Gene ID81631
Swiss protQ9GZQ8
SynonymsLC3B; ATG8F; MAP1LC3B-a; MAP1A/1BLC3; MAP1LC3B; LC3A/LC3B
Calculated MW14kDa
Observed MW14KDa/16KDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC 1:50 - 1:200
  • IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samples293T, NIH/3T3, C6
Cellular locationCytoplasm, Cytoplasmic vesicle, Endomembrane system, Lipid-anchor, autophagosome, autophagosome membrane, cytoskeleton
Customer validation

WB(Sus scrofa, Homo sapiens, Mus musculus, Rattus norvegicus)

IF(Homo sapiens)

IHC(Mus musculus)

IF(Mus musculus)

IHC(Rattus norvegicus)

ABclonal:Western blot - [KO Validated] LC3B Rabbit pAb (A11282)}

Western blot - [KO Validated] LC3B Rabbit pAb (A11282)

Western blot analysis of extracts of various cell lines, using LC3B antibody (A11282) at 1:500 dilution.NIH/3T3 cells were treated by Chloroquine (50 μM) at 37℃ for 20 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Western blot - [KO Validated] LC3B Rabbit pAb (A11282)}

Western blot - [KO Validated] LC3B Rabbit pAb (A11282)

Western blot analysis of extracts of 293T cells, using LC3B antibody (A11282) at 1:500 dilution.293T cells were treated by Chloroquine (50 μM) at 37℃ for 20 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunohistochemistry - [KO Validated] LC3B Rabbit pAb (A11282)}

Immunohistochemistry - [KO Validated] LC3B Rabbit pAb (A11282)

Immunohistochemistry of paraffin-embedded mouse brain using [KO Validated] LC3B Rabbit pAb (A11282) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - [KO Validated] LC3B Rabbit pAb (A11282)}

Immunohistochemistry - [KO Validated] LC3B Rabbit pAb (A11282)

Immunohistochemistry of paraffin-embedded rat brain using [KO Validated] LC3B Rabbit pAb (A11282) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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