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[KO Validated] LC3B Rabbit pAb (A11282)

Publications (9) Datasheet

ABclonal:Western blot - [KO Validated] LC3B Rabbit pAb (A11282)
ABclonal:Western blot - [KO Validated] LC3B Rabbit pAb (A11282)
ABclonal:Immunofluorescence - [KO Validated] LC3B Rabbit pAb (A11282)

Overview

Product name[KO Validated] LC3B Rabbit pAb
Catalog No.A11282
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component.
ImmunogenA synthetic peptide of human LC3B
SequenceEmail for sequence
Gene ID81631
Swiss protQ9GZQ8
SynonymsLC3B; ATG8F; MAP1LC3B-a; MAP1A/1BLC3; MAP1LC3B; LC3A/LC3B
Calculated MW14kDa
Observed MW16kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunofluorescence    
Positive samplesC6, NIH/3T3, Mouse brain, Rat brain
Cellular locationCytoplasm, Cytoplasmic vesicle, Endomembrane system, Lipid-anchor, autophagosome, autophagosome membrane, cytoskeleton
Customer validation

IF(Homo sapiens)

WB(Homo sapiens, Sus scrofa, Mus musculus)

ABclonal:Western blot - [KO Validated] LC3B Rabbit pAb (A11282) }

Western blot - [KO Validated] LC3B Rabbit pAb (A11282)

Western blot analysis of extracts from normal (control) and LC3B knockout (KO) 293T cells, using LC3B antibody (A11282) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Western blot - [KO Validated] LC3B Rabbit pAb (A11282) }

Western blot - [KO Validated] LC3B Rabbit pAb (A11282)

Western blot analysis of extracts of various cell lines, using LC3B antibody (A11282) at 1:1000 dilution. Both C6 cells and NIH/3T3 cells were treated by Chloroquine (50 μM) at 37℃ for 20 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3min.
ABclonal:Immunofluorescence - [KO Validated] LC3B Rabbit pAb (A11282) }

Immunofluorescence - [KO Validated] LC3B Rabbit pAb (A11282)

Immunofluorescence analysis of NIH-3T3 treated by chloroquine and NIH-3T3 cells using [KO Validated] LC3B Rabbit pAb (A11282) at dilution of 100 (40x lens).NIH/3T3 cells were treated by Chloroquine (50 μM) for 20 hours(left). Blue: DAPI for nuclear staining.

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