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[KO Validated] LC3B Rabbit mAb (A19665)

Review (1) Datasheet

ABclonal:Western blot - [KO Validated] LC3B Rabbit mAb (A19665)
ABclonal:Western blot - [KO Validated] LC3B Rabbit mAb (A19665)
ABclonal:Immunohistochemistry - [KO Validated] LC3B Rabbit mAb (A19665)
ABclonal:Immunohistochemistry - [KO Validated] LC3B Rabbit mAb (A19665)
ABclonal:Immunohistochemistry - [KO Validated] LC3B Rabbit mAb (A19665)
ABclonal:Immunofluorescence - [KO Validated] LC3B Rabbit mAb (A19665)
ABclonal:Immunofluorescence - [KO Validated] LC3B Rabbit mAb (A19665)
ABclonal:Immunofluorescence - [KO Validated] LC3B Rabbit mAb (A19665)
Review
ABclonal: review for [KO Validated] LC3B Rabbit mAb(A19665)

Overview

Product name[KO Validated] LC3B Rabbit mAb
Catalog No.A19665
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component.
ImmunogenA synthetic peptide of human LC3B.
SequenceEmail for sequence
Gene ID81631
Swiss protQ9GZQ8
SynonymsLC3B; ATG8F; MAP1LC3B-a; MAP1A/1BLC3; MAP1LC3B; LC3A/LC3B
Calculated MW14kDa, 16kDa
Observed MW14kDa, 16kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 1% BSA, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesHeLa, 293T, C6, NIH/3T3
Cellular locationCytoplasm, Cytoplasmic vesicle, Endomembrane system, Lipid-anchor, autophagosome, autophagosome membrane, cytoskeleton
Customer validation

(Chicken)

ABclonal:Western blot - [KO Validated] LC3B Rabbit mAb (A19665) }

Western blot - [KO Validated] LC3B Rabbit mAb (A19665)

Western blot analysis of extracts of various cell lines, using LC3B antibody (A19665) at 1:1000 dilution.293T cells, LC3B knockout (KO) 293T cells, C6 cells and NIH/3T3 cells were treated by Chloroquine (50 μM) at 37℃ for 20 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.
ABclonal:Western blot - [KO Validated] LC3B Rabbit mAb (A19665) }

Western blot - [KO Validated] LC3B Rabbit mAb (A19665)

Western blot analysis of extracts of Hela cells, using LC3B pAb (A19665) at 1:1000 dilution.Hela cells were treated by Chloroquine (50 μM) at 37℃ for 20 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.
ABclonal:Immunohistochemistry - [KO Validated] LC3B Rabbit mAb (A19665) }

Immunohistochemistry - [KO Validated] LC3B Rabbit mAb (A19665)

Immunohistochemistry of paraffin-embedded Rat brain using LC3B antibody (A19665) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - [KO Validated] LC3B Rabbit mAb (A19665) }

Immunohistochemistry - [KO Validated] LC3B Rabbit mAb (A19665)

Immunohistochemistry of paraffin-embedded Human brain using LC3B antibody (A19665) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - [KO Validated] LC3B Rabbit mAb (A19665) }

Immunohistochemistry - [KO Validated] LC3B Rabbit mAb (A19665)

Immunohistochemistry of paraffin-embedded Mouse spinal cord using LC3B antibody (A19665) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - [KO Validated] LC3B Rabbit mAb (A19665) }

Immunofluorescence - [KO Validated] LC3B Rabbit mAb (A19665)

Immunofluorescence analysis of C6 cells, untreated(right) and Chloroquine treated(50 μM, 37℃ for 20 hours;left) using LC3B antibody (A19665) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - [KO Validated] LC3B Rabbit mAb (A19665) }

Immunofluorescence - [KO Validated] LC3B Rabbit mAb (A19665)

Immunofluorescence analysis of HeLa cells, untreated(right) and Chloroquine treated(50 μM, 37℃ for 20 hours;left) using LC3B antibody (A19665) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - [KO Validated] LC3B Rabbit mAb (A19665) }

Immunofluorescence - [KO Validated] LC3B Rabbit mAb (A19665)

Immunofluorescence analysis of NIH/3T3 cells, untreated(right) and Chloroquine treated(50 μM, 37℃ for 20 hours;left) using LC3B antibody (A19665) at dilution of 1:100. Blue: DAPI for nuclear staining.

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