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[KO Validated] LC3B Rabbit pAb (A7198)

Reviews (11)Publications (21) Datasheet

ABclonal:[KO Validated] LC3B Rabbit pAb
ABclonal:Western blot - [KO Validated] LC3B Rabbit pAb (A7198)

Western blot analysis of extracts of various cell lines, using LC3B antibody (A7198) at 1:1000 dilution. C6 cells were treated by Chloroquine (50 μM) for 20 hours. Hela cells were treated by Chloroquine (50 μM) for 20 hours. NIH/3T3 cells were treated by Chloroquine (50 μM) for 20 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Western blot - [KO Validated] LC3B Rabbit pAb (A7198)

Western blot analysis of extracts of pig liver, using LC3B antibody (A7198).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

ABclonal:Western blot - [KO Validated] LC3B Rabbit pAb (A7198)

Western blot analysis of extracts from normal (control) and LC3B knockout (KO) 293T cells, using LC3B antibody (A7198) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunohistochemistry - [KO Validated] LC3B Rabbit pAb (A7198)

Immunohistochemistry of paraffin-embedded rat brain using LC3B antibody (A7198) at dilution of 1:100 (40x lens).

ABclonal:Immunohistochemistry - [KO Validated] LC3B Rabbit pAb (A7198)

Immunohistochemistry of paraffin-embedded human breast cancer using LC3B antibody (A7198) at dilution of 1:100 (40x lens).

ABclonal:Immunohistochemistry - [KO Validated] LC3B Rabbit pAb (A7198)

Immunohistochemistry of paraffin-embedded human stomach using LC3B antibody (A7198) at dilution of 1:100 (40x lens).

ABclonal:Immunofluorescence - [KO Validated] LC3B Rabbit pAb (A7198)

Immunofluorescence analysis of rat brain using LC3B antibody (A7198) at dilution of 1:100. Blue: DAPI for nuclear staining.

Review
ABclonal: review for [KO Validated] LC3B Rabbit pAb(A7198)
Review
ABclonal: review for [KO Validated] LC3B Rabbit pAb(A7198)
Review
ABclonal: review for [KO Validated] LC3B Rabbit pAb(A7198)
Review
ABclonal: review for [KO Validated] LC3B Rabbit pAb(A7198)
Review
ABclonal: review for [KO Validated] LC3B Rabbit pAb(A7198)
Review
ABclonal: review for [KO Validated] LC3B Rabbit pAb(A7198)
Review
ABclonal: review for [KO Validated] LC3B Rabbit pAb(A7198)
Review
ABclonal: review for [KO Validated] LC3B Rabbit pAb(A7198)
Review
ABclonal: review for [KO Validated] LC3B Rabbit pAb(A7198)
Review
ABclonal: review for [KO Validated] LC3B Rabbit pAb(A7198)
Review
ABclonal: review for [KO Validated] LC3B Rabbit pAb(A7198)

Overview

Product name[KO Validated] LC3B Rabbit pAb
Catalog No.A7198
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component.
ImmunogenA synthetic peptide of human LC3B
SequenceEmail for sequence
Gene ID81631
Swiss protQ9GZQ8
SynonymsLC3B; ATG8F; MAP1LC3B-a; MAP1A/1BLC3; MAP1LC3B; LC3A/LC3B
Calculated MW14kDa
Observed MW14kDa, 16kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesC6, HeLa, NIH/3T3, Pig liver
Cellular locationCytoplasm, Cytoplasmic vesicle, Endomembrane system, Lipid-anchor, autophagosome, autophagosome membrane, cytoskeleton
Customer validation

(MSC cells, Human lung cancer cell, C2C12, Squamous cell carcinoma, Mouse cells, 293T, U87MG, Rat cells)

WB(Homo sapiens, Mus musculus, Rattus norvegicus, Danio rerio, Sus scrofa, Bombyx mori)

IF(Homo sapiens)

IHC(Homo sapiens, Mus musculus)

IF(Mus musculus, Homo sapiens)

ABclonal:Western blot - [KO Validated] LC3B Rabbit pAb (A7198) }

Western blot - [KO Validated] LC3B Rabbit pAb (A7198)

Western blot analysis of extracts of various cell lines, using LC3B antibody (A7198) at 1:1000 dilution. C6 cells were treated by Chloroquine (50 μM) for 20 hours. Hela cells were treated by Chloroquine (50 μM) for 20 hours. NIH/3T3 cells were treated by Chloroquine (50 μM) for 20 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Western blot - [KO Validated] LC3B Rabbit pAb (A7198) }

Western blot - [KO Validated] LC3B Rabbit pAb (A7198)

Western blot analysis of extracts of pig liver, using LC3B antibody (A7198).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
ABclonal:Western blot - [KO Validated] LC3B Rabbit pAb (A7198) }

Western blot - [KO Validated] LC3B Rabbit pAb (A7198)

Western blot analysis of extracts from normal (control) and LC3B knockout (KO) 293T cells, using LC3B antibody (A7198) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunohistochemistry - [KO Validated] LC3B Rabbit pAb (A7198) }

Immunohistochemistry - [KO Validated] LC3B Rabbit pAb (A7198)

Immunohistochemistry of paraffin-embedded rat brain using LC3B antibody (A7198) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - [KO Validated] LC3B Rabbit pAb (A7198) }

Immunohistochemistry - [KO Validated] LC3B Rabbit pAb (A7198)

Immunohistochemistry of paraffin-embedded human breast cancer using LC3B antibody (A7198) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - [KO Validated] LC3B Rabbit pAb (A7198) }

Immunohistochemistry - [KO Validated] LC3B Rabbit pAb (A7198)

Immunohistochemistry of paraffin-embedded human stomach using LC3B antibody (A7198) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - [KO Validated] LC3B Rabbit pAb (A7198) }

Immunofluorescence - [KO Validated] LC3B Rabbit pAb (A7198)

Immunofluorescence analysis of rat brain using LC3B antibody (A7198) at dilution of 1:100. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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