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[KO Validated] L1CAM Rabbit pAb (A8555)

Publication (1) Datasheet

ABclonal:Western blot - [KO Validated] L1CAM Polyclonal Antibody (A8555)
ABclonal:Western blot - [KO Validated] L1CAM Polyclonal Antibody (A8555)

Overview

Product name[KO Validated] L1CAM Rabbit pAb
Catalog No.A8555
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is an axonal glycoprotein belonging to the immunoglobulin supergene family. The ectodomain, consisting of several immunoglobulin-like domains and fibronectin-like repeats (type III), is linked via a single transmembrane sequence to a conserved cytoplasmic domain. This cell adhesion molecule plays an important role in nervous system development, including neuronal migration and differentiation. Mutations in the gene cause X-linked neurological syndromes known as CRASH (corpus callosum hypoplasia, retardation, aphasia, spastic paraplegia and hydrocephalus). Alternative splicing of this gene results in multiple transcript variants, some of which include an alternate exon that is considered to be specific to neurons.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1143-1257 of human L1CAM (NP_000416.1).
SequenceIKRSKGGKYSVKDKEDTQVDSEARPMKDETFGEYRSLESDNEEKAFGSSQPSLNGDIKPLGSDDSLADYGGSVDVQFNEDGSFIGQYSGKKEKEAAGGNDSSGATSPINPAVALE
Gene ID3897
Swiss protP32004
SynonymsL1CAM; CAML1; CD171; HSAS; HSAS1; MASA; MIC5; N-CAM-L1; N-CAML1; NCAM-L1; S10; SPG1
Calculated MW138kDa/139kDa/140kDa
Observed MW200kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    
Positive samplesSH-SY5Y, Mouse brain
Cellular locationCell membrane, Cell projection, Single-pass type I membrane protein, growth cone
Customer validation

WB(Homo sapiens)

ABclonal:Western blot - [KO Validated] L1CAM Polyclonal Antibody (A8555) }

Western blot - [KO Validated] L1CAM Polyclonal Antibody (A8555)

Western blot analysis of extracts of various cell lines, using L1CAM antibody (A8555) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Western blot - [KO Validated] L1CAM Polyclonal Antibody (A8555) }

Western blot - [KO Validated] L1CAM Polyclonal Antibody (A8555)

Western blot analysis of extracts from normal (control) and L1CAM knockout (KO) HeLa cells, using L1CAM antibody (A8555) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

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