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[KO Validated] Cytokeratin 8 Rabbit pAb (A1024)

Publication (1) Datasheet

ABclonal:Western blot - [KO Validated] Cytokeratin 8 Rabbit pAb (A1024)
ABclonal:Western blot - [KO Validated] Cytokeratin 8 Rabbit pAb (A1024)
ABclonal:Immunohistochemistry - [KO Validated] Cytokeratin 8 Rabbit pAb (A1024)
ABclonal:Immunofluorescence - [KO Validated] Cytokeratin 8 Rabbit pAb (A1024)

Overview

Product name[KO Validated] Cytokeratin 8 Rabbit pAb
Catalog No.A1024
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis. Alternatively spliced transcript variants have been found for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-483 of human Cytokeratin 8 (NP_002264.1).
SequenceMSIRVTQKSYKVSTSGPRAFSSRSYTSGPGSRISSSSFSRVGSSNFRGGLGGGYGGASGMGGITAVTVNQSLLSPLVLEVDPNIQAVRTQEKEQIKTLNNKFASFIDKVRFLEQQNKMLETKWSLLQQQKTARSNMDNMFESYINNLRRQLETLGQEKLKLEAELGNMQGLVEDFKNKYEDEINKRTEMENEFVLIKKDVDEAYMNKVELESRLEGLTDEINFLRQLYEEEIRELQSQISDTSVVLSMDNSRSLDMDSIIAEVKAQYEDIANRSRAEAESMYQIKYEELQSLAGKHGDDLRRTKTEISEMNRNISRLQAEIEGLKGQRASLEAAIADAEQRGELAIKDANAKLSELEAALQRAKQDMARQLREYQELMNVKLALDIEIATYRKLLEGEESRLESGMQNMSIHTKTTSGYAGGLSSAYGGLTSPGLSYSLGSSFGSGAGSSSFSRTSSSRAVVVKKIETRDGKLVSESSDVLPK
Gene ID3856
Swiss protP05787
SynonymsKRT8; CARD2; CK-8; CK8; CYK8; K2C8; K8; KO; keratin 8
Calculated MW53kDa/56kDa
Observed MW53kDa
ReactivityHuman, Mouse
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samples293T, NIH/3T3
Cellular locationCytoplasm, Nucleus, Nucleus matrix, nucleoplasm
Customer validation

WB(Mus musculus)

ABclonal:Western blot - [KO Validated] Cytokeratin 8 Rabbit pAb (A1024) }

Western blot - [KO Validated] Cytokeratin 8 Rabbit pAb (A1024)

Western blot analysis of extracts of various cell lines, using Cytokeratin 8 antibody (A1024) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
ABclonal:Western blot - [KO Validated] Cytokeratin 8 Rabbit pAb (A1024) }

Western blot - [KO Validated] Cytokeratin 8 Rabbit pAb (A1024)

Western blot analysis of extracts from normal (control) and Cytokeratin 8 knockout (KO) HeLa cells, using Cytokeratin 8 antibody (A1024) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Immunohistochemistry - [KO Validated] Cytokeratin 8 Rabbit pAb (A1024) }

Immunohistochemistry - [KO Validated] Cytokeratin 8 Rabbit pAb (A1024)

Immunohistochemistry of paraffin-embedded human appendix using [KO Validated] Cytokeratin 8 Rabbit pAb (A1024) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - [KO Validated] Cytokeratin 8 Rabbit pAb (A1024) }

Immunofluorescence - [KO Validated] Cytokeratin 8 Rabbit pAb (A1024)

Immunofluorescence analysis of HeLa cells using Cytokeratin 8 antibody (A1024) at dilution of 1:100. Blue: DAPI for nuclear staining.

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