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[KO Validated] KDM5B Rabbit pAb (A7772)

Review (1) Datasheet

ABclonal:Western blot - [KO Validated] KDM5B Rabbit pAb (A7772)

Western blot analysis of extracts of 293T cells, using KDM5B antibody (A7772) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

Review
ABclonal: review for [KO Validated] KDM5B Rabbit pAb(A7772)

Overview

Product name[KO Validated] KDM5B Rabbit pAb
Catalog No.A7772
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a lysine-specific histone demethylase that belongs to the jumonji/ARID domain-containing family of histone demethylases. The encoded protein is capable of demethylating tri-, di- and monomethylated lysine 4 of histone H3. This protein plays a role in the transcriptional repression or certain tumor suppressor genes and is upregulated in certain cancer cells. This protein may also play a role in genome stability and DNA repair. Alternate splicing results in multiple transcript variants.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 784-883 of human KDM5B (NP_006609.3).
SequenceEESEMKKFPDNDLLRHLRLVTQDAEKCASVAQQLLNGKRQTRYRSGGGKSQNQLTVNELRQFVTQLYALPCVLSQTPLLKDLLNRVEDFQQHSQKLLSEE
Gene ID10765
Swiss protQ9UGL1
SynonymsKDM5B; CT31; JARID1B; PLU-1; PLU1; PPP1R98; PUT1; RBBP2H1A; RBP2-H1
Calculated MW175kDa/179kDa
Observed MW176KDa
ReactivityHuman
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunofluorescence    
Positive samples293T
Cellular locationNucleus
Customer validation

(Human hepatocellular carcinoma cell line)

ABclonal:Western blot - [KO Validated] KDM5B Rabbit pAb (A7772) }

Western blot - [KO Validated] KDM5B Rabbit pAb (A7772)

Western blot analysis of extracts of 293T cells, using KDM5B antibody (A7772) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

* For research use only. Not for therapeutic or diagnostic purposes.

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