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[KO Validated] KDM1 Rabbit pAb (A15794)

Publication (1) Datasheet

ABclonal:Western blot - [KO Validated] KDM1 Rabbit pAb (A15794)

Western blot analysis of extracts of various cell lines, using KDM1 antibody (A15794) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Western blot - [KO Validated] KDM1 Rabbit pAb (A15794)

Western blot analysis of extracts from normal (control) and KDM1 Rabbit pAb knockout (KO) 293T cells, using KDM1 Rabbit pAb antibody (A15794) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Immunohistochemistry - [KO Validated] KDM1 Rabbit pAb (A15794)

Immunohistochemistry of paraffin-embedded rat spleen using KDM1 antibody (A15794) at dilution of 1:100 (40x lens).

ABclonal:Immunohistochemistry - [KO Validated] KDM1 Rabbit pAb (A15794)

Immunohistochemistry of paraffin-embedded human esophageal using KDM1 antibody (A15794) at dilution of 1:100 (40x lens).

ABclonal:Immunohistochemistry - [KO Validated] KDM1 Rabbit pAb (A15794)

Immunohistochemistry of paraffin-embedded mouse brain using KDM1 antibody (A15794) at dilution of 1:100 (40x lens).

ABclonal:Immunofluorescence - [KO Validated] KDM1 Rabbit pAb (A15794)

Immunofluorescence analysis of HeLa cells using KDM1 Polyclonal Antibody (A15794) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - [KO Validated] KDM1 Rabbit pAb (A15794)

Immunofluorescence analysis of L929 cells using KDM1 Polyclonal Antibody (A15794) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - [KO Validated] KDM1 Rabbit pAb (A15794)

Confocal immunofluorescence analysis of Hela cells using KDM1 Polyclonal Antibody (A15794) at dilution of 1:200. Blue: DAPI for nuclear staining.

Overview

Product name[KO Validated] KDM1 Rabbit pAb
Catalog No.A15794
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a nuclear protein containing a SWIRM domain, a FAD-binding motif, and an amine oxidase domain. This protein is a component of several histone deacetylase complexes, though it silences genes by functioning as a histone demethylase. Alternative splicing results in multiple transcript variants.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 130-380 of human KDM1 (NP_055828.2).
SequenceLSEDEYYSEEERNAKAEKEKKLPPPPPQAPPEEENESEPEEPSGVEGAAFQSRLPHDRMTSQEAACFPDIISGPQQTQKVFLFIRNRTLQLWLDNPKIQLTFEATLQQLEAPYNSDTVLVHRVHSYLERHGLINFGIYKRIKPLPTKKTGKVIIIGSGVSGLAAARQLQSFGMDVTLLEARDRVGGRVATFRKGNYVADLGAMVVTGLGGNPMAVVSKQVNMELAKIKQKCPLYEANGQAVPKEKDEMVEQ
Gene ID23028
Swiss protO60341
SynonymsKDM1A; AOF2; BHC110; CPRF; KDM1; LSD1; KDM1
Calculated MW92kDa/95kDa
Observed MW110kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:100
IF 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesU-87MG, HeLa, Jurkat, Mouse liver, 293T
Cellular locationNucleus
Customer validation

WB(Homo sapiens)

ABclonal:Western blot - [KO Validated] KDM1 Rabbit pAb (A15794) }

Western blot - [KO Validated] KDM1 Rabbit pAb (A15794)

Western blot analysis of extracts of various cell lines, using KDM1 antibody (A15794) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Western blot - [KO Validated] KDM1 Rabbit pAb (A15794) }

Western blot - [KO Validated] KDM1 Rabbit pAb (A15794)

Western blot analysis of extracts from normal (control) and KDM1 Rabbit pAb knockout (KO) 293T cells, using KDM1 Rabbit pAb antibody (A15794) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
ABclonal:Immunohistochemistry - [KO Validated] KDM1 Rabbit pAb (A15794) }

Immunohistochemistry - [KO Validated] KDM1 Rabbit pAb (A15794)

Immunohistochemistry of paraffin-embedded rat spleen using KDM1 antibody (A15794) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - [KO Validated] KDM1 Rabbit pAb (A15794) }

Immunohistochemistry - [KO Validated] KDM1 Rabbit pAb (A15794)

Immunohistochemistry of paraffin-embedded human esophageal using KDM1 antibody (A15794) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - [KO Validated] KDM1 Rabbit pAb (A15794) }

Immunohistochemistry - [KO Validated] KDM1 Rabbit pAb (A15794)

Immunohistochemistry of paraffin-embedded mouse brain using KDM1 antibody (A15794) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - [KO Validated] KDM1 Rabbit pAb (A15794) }

Immunofluorescence - [KO Validated] KDM1 Rabbit pAb (A15794)

Immunofluorescence analysis of HeLa cells using KDM1 Polyclonal Antibody (A15794) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - [KO Validated] KDM1 Rabbit pAb (A15794) }

Immunofluorescence - [KO Validated] KDM1 Rabbit pAb (A15794)

Immunofluorescence analysis of L929 cells using KDM1 Polyclonal Antibody (A15794) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - [KO Validated] KDM1 Rabbit pAb (A15794) }

Immunofluorescence - [KO Validated] KDM1 Rabbit pAb (A15794)

Confocal immunofluorescence analysis of Hela cells using KDM1 Polyclonal Antibody (A15794) at dilution of 1:200. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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