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[KO Validated] KDM1 Polyclonal Antibody (A1156)

Publication (1) Datasheet

ABclonal:Western blot - [KO Validated] KDM1 Polyclonal Antibody (A1156)
ABclonal:Western blot - [KO Validated] KDM1 Polyclonal Antibody (A1156)
ABclonal:Immunofluorescence - [KO Validated] KDM1 Polyclonal Antibody (A1156)
ABclonal:Immunoprecipitation - [KO Validated] KDM1 Polyclonal Antibody (A1156)

Overview

Product name[KO Validated] KDM1 Polyclonal Antibody
Catalog No.A1156
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a nuclear protein containing a SWIRM domain, a FAD-binding motif, and an amine oxidase domain. This protein is a component of several histone deacetylase complexes, though it silences genes by functioning as a histone demethylase. Alternative splicing results in multiple transcript variants.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 130-380 of human KDM1 (NP_055828.2).
SequenceLSEDEYYSEEERNAKAEKEKKLPPPPPQAPPEEENESEPEEPSGVEGAAFQSRLPHDRMTSQEAACFPDIISGPQQTQKVFLFIRNRTLQLWLDNPKIQLTFEATLQQLEAPYNSDTVLVHRVHSYLERHGLINFGIYKRIKPLPTKKTGKVIIIGSGVSGLAAARQLQSFGMDVTLLEARDRVGGRVATFRKGNYVADLGAMVVTGLGGNPMAVVSKQVNMELAKIKQKCPLYEANGQAVPKEKDEMVEQ
Gene ID23028
Swiss protO60341
SynonymsKDM1A; AOF2; BHC110; CPRF; KDM1; LSD1; lysine demethylase 1A
Calculated MW92kDa/95kDa
Observed MW96-117kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide Array
Recommended dilutionWB 1:500 - 1:2000
IF 1:50 - 1:100
IP 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunofluorescence    Immunoprecipitation    
Positive samplesPC-12, MCF7
Cellular locationNucleus
Customer validation

WB(Human)

ABclonal:Western blot - [KO Validated] KDM1 Polyclonal Antibody (A1156) }

Western blot - [KO Validated] KDM1 Polyclonal Antibody (A1156)

Western blot analysis of extracts of various cell lines, using KDM1 antibody (A1156) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
ABclonal:Western blot - [KO Validated] KDM1 Polyclonal Antibody (A1156) }

Western blot - [KO Validated] KDM1 Polyclonal Antibody (A1156)

Western blot analysis of extracts from normal (control) and KDM1 knockout (KO) HeLa cells, using KDM1 antibody (A1156) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunofluorescence - [KO Validated] KDM1 Polyclonal Antibody (A1156) }

Immunofluorescence - [KO Validated] KDM1 Polyclonal Antibody (A1156)

Immunofluorescence analysis of A549 cells using KDM1 antibody (A1156).
ABclonal:Immunoprecipitation - [KO Validated] KDM1 Polyclonal Antibody (A1156) }

Immunoprecipitation - [KO Validated] KDM1 Polyclonal Antibody (A1156)

Immunoprecipitation analysis of 200ug extracts of HeLa cells using 3ug KDM1 antibody (A1156). Western blot was performed from the immunoprecipitate using KDM1 antibody (A1156) at a dilition of 1:500.

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