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[KO Validated] ITGA2 Rabbit pAb (A7629)

Datasheet

ABclonal:Western blot - [KO Validated] ITGA2 Polyclonal Antibody (A7629)
ABclonal:Western blot - [KO Validated] ITGA2 Polyclonal Antibody (A7629)
ABclonal:Immunohistochemistry - [KO Validated] ITGA2 Polyclonal Antibody (A7629)
ABclonal:Immunohistochemistry - [KO Validated] ITGA2 Polyclonal Antibody (A7629)
ABclonal:Immunofluorescence - [KO Validated] ITGA2 Polyclonal Antibody (A7629)

Overview

Product name[KO Validated] ITGA2 Rabbit pAb
Catalog No.A7629
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes the alpha subunit of a transmembrane receptor for collagens and related proteins. The encoded protein forms a heterodimer with a beta subunit and mediates the adhesion of platelets and other cell types to the extracellular matrix. Loss of the encoded protein is associated with bleeding disorder platelet-type 9. Antibodies against this protein are found in several immune disorders, including neonatal alloimmune thrombocytopenia. This gene is located adjacent to a related alpha subunit gene. Alternative splicing results in multiple transcript variants.
ImmunogenRecombinant protein of human ITGA2
SequenceEmail for sequence
Gene ID3673
Swiss protP17301
SynonymsBR; CD49B; GPIa; HPA-5; VLA-2; VLAA2; ITGA2
Calculated MW129kDa
Observed MW150kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
IHC 1:100 - 1:200
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesHeLa, HT-1080
Cellular locationMembrane, Single-pass type I membrane protein
ABclonal:Western blot - [KO Validated] ITGA2 Polyclonal Antibody (A7629) }

Western blot - [KO Validated] ITGA2 Polyclonal Antibody (A7629)

Western blot analysis of extracts of various cell lines, using ITGA2 antibody (A7629) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 15s.
ABclonal:Western blot - [KO Validated] ITGA2 Polyclonal Antibody (A7629) }

Western blot - [KO Validated] ITGA2 Polyclonal Antibody (A7629)

Western blot analysis of extracts from normal (control) and ITGA2 knockout (KO) HeLa cells, using ITGA2 antibody (A7629) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunohistochemistry - [KO Validated] ITGA2 Polyclonal Antibody (A7629) }

Immunohistochemistry - [KO Validated] ITGA2 Polyclonal Antibody (A7629)

Immunohistochemistry of paraffin-embedded rat brain using ITGA2 Antibody (A7629) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - [KO Validated] ITGA2 Polyclonal Antibody (A7629) }

Immunohistochemistry - [KO Validated] ITGA2 Polyclonal Antibody (A7629)

Immunohistochemistry of paraffin-embedded mouse brain using ITGA2 Antibody (A7629) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - [KO Validated] ITGA2 Polyclonal Antibody (A7629) }

Immunofluorescence - [KO Validated] ITGA2 Polyclonal Antibody (A7629)

Immunofluorescence analysis of U2OS cells using ITGA2 antibody (A7629) at dilution of 1:100. Blue: DAPI for nuclear staining.

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