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[KO Validated] IRF3 Polyclonal Antibody (A11118)

Publication (1) Datasheet

ABclonal:Western blot - [KO Validated] IRF3 Polyclonal Antibody (A11118)
ABclonal:Western blot - [KO Validated] IRF3 Polyclonal Antibody (A11118)
ABclonal:Immunohistochemistry - [KO Validated] IRF3 Polyclonal Antibody (A11118)
ABclonal:Immunohistochemistry - [KO Validated] IRF3 Polyclonal Antibody (A11118)
ABclonal:Chromatin Immunoprecipitation - [KO Validated] IRF3 Polyclonal Antibody (A11118)

Overview

Product name[KO Validated] IRF3 Polyclonal Antibody
Catalog No.A11118
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the interferon regulatory transcription factor (IRF) family. The encoded protein is found in an inactive cytoplasmic form that upon serine/threonine phosphorylation forms a complex with CREBBP. This complex translocates to the nucleus and activates the transcription of interferons alpha and beta, as well as other interferon-induced genes. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human IRF3 (NP_001184051.1).
SequenceMGTPKPRILPWLVSQLDLGQLEGVAWVNKSRTRFRIPWKHGLRQDAQQEDFGIFQAWAEATGAYVPGRDKPDLPTWKRNFRSALNRKEGLRLAEDRSKDPHDPHKIYEFVNSGVGDFSQPDTSPDTNGGGSTSDTQEDILDELLGNMVLAPLPDPGPPSLAVAPEPCPQPLRSPSLDNPTPFPNLGPSENPLKRLLVPGEEWEFEVTAFYRGRQVFQQTISCPEGLRLVGSEVGDRTLPGWPVTLPDPGMSLTDRGVMSY
Gene ID3661
Swiss protQ14653
SynonymsIRF3; IIAE7; interferon regulatory factor 3
Calculated MW12kDa/16kDa/33kDa/47kDa/49kDa
Observed MW56kDa
ReactivityHuman, Mouse
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide Array
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
ChIP 1:20 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samples293T, HeLa, Jurkat, HepG2
Cellular locationCytoplasm, Nucleus
Customer validation

WB(Human)

ABclonal:Western blot - [KO Validated] IRF3 Polyclonal Antibody (A11118) }

Western blot - [KO Validated] IRF3 Polyclonal Antibody (A11118)

Western blot analysis of extracts of various cell lines, using IRF3 antibody (A11118) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Western blot - [KO Validated] IRF3 Polyclonal Antibody (A11118) }

Western blot - [KO Validated] IRF3 Polyclonal Antibody (A11118)

Western blot analysis of extracts from normal (control) and IRF3 knockout (KO) HeLa cells, using IRF3 antibody (A11118) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.
ABclonal:Immunohistochemistry - [KO Validated] IRF3 Polyclonal Antibody (A11118) }

Immunohistochemistry - [KO Validated] IRF3 Polyclonal Antibody (A11118)

Immunohistochemistry of paraffin-embedded human lung cancer using IRF3 antibody (A11118) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - [KO Validated] IRF3 Polyclonal Antibody (A11118) }

Immunohistochemistry - [KO Validated] IRF3 Polyclonal Antibody (A11118)

Immunohistochemistry of paraffin-embedded human esophageal cancer using IRF3 antibody (A11118) at dilution of 1:100 (40x lens).
ABclonal:Chromatin Immunoprecipitation - [KO Validated] IRF3 Polyclonal Antibody (A11118) }

Chromatin Immunoprecipitation - [KO Validated] IRF3 Polyclonal Antibody (A11118)

Chromatin immunoprecipitation analysis of extracts of HCT116 cell line, using IRF3 rabbit polyclonal antibody (A11118) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

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