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[KO Validated] IL18 Polyclonal Antibody (A1115)

Reviews (2)Publications (2) Datasheet

ABclonal:Western blot - [KO Validated] IL18 Polyclonal Antibody (A1115)
ABclonal:Western blot - [KO Validated] IL18 Polyclonal Antibody (A1115)
ABclonal:Immunoprecipitation - [KO Validated] IL18 Polyclonal Antibody (A1115)
Review
ABclonal:Western blot (WB) review for [KO Validated] IL18 Polyclonal Antibody(A1115)
Review
ABclonal:Western blot (WB) review for [KO Validated] IL18 Polyclonal Antibody(A1115)

Overview

Product name[KO Validated] IL18 Polyclonal Antibody
Catalog No.A1115
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a proinflammatory cytokine that augments natural killer cell activity in spleen cells, and stimulates interferon gamma production in T-helper type I cells. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 37-193 of human IL18 (NP_001553.1).
SequenceYFGKLESKLSVIRNLNDQVLFIDQGNRPLFEDMTDSDCRDNAPRTIFIISMYKDSQPRGMAVTISVKCEKISTLSCENKIISFKEMNPPDNIKDTKSDIIFFQRSVPGHDNKMQFESSSYEGYFLACEKERDLFKLILKKEDELGDRSIMFTVQNED
Gene ID3606
Swiss protQ14116
SynonymsIL18; IGIF; IL-18; IL-1g; IL1F4; interleukin-18
Calculated MW21kDa/22kDa
Observed MW23kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFC
Recommended dilutionWB 1:500 - 1:2000
IP 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunoprecipitation    
Positive samplesHeLa, Mouse lung
Cellular locationSecreted
Customer validation

WB(N9 cells, HUVEC cells, Mouse, human)

ABclonal:Western blot - [KO Validated] IL18 Polyclonal Antibody (A1115) }

Western blot - [KO Validated] IL18 Polyclonal Antibody (A1115)

Western blot analysis of extracts of various cell lines, using IL18 antibody (A1115) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
ABclonal:Western blot - [KO Validated] IL18 Polyclonal Antibody (A1115) }

Western blot - [KO Validated] IL18 Polyclonal Antibody (A1115)

Western blot analysis of extracts from normal (control) and IL18 knockout (KO) HeLa cells, using IL18 antibody (A1115) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.
ABclonal:Immunoprecipitation - [KO Validated] IL18 Polyclonal Antibody (A1115) }

Immunoprecipitation - [KO Validated] IL18 Polyclonal Antibody (A1115)

Immunoprecipitation analysis of 200ug extracts of HeLa cells, using 3 ug IL18 antibody (A1115). Western blot was performed from the immunoprecipitate using IL18 antibody (A1115) at a dilition of 1:1000.

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