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[KO Validated] IKKγ Rabbit pAb (A12536)

Datasheet

ABclonal:Western blot - [KO Validated] IKKγ Rabbit pAb (A12536)
ABclonal:Western blot - [KO Validated] IKKγ Rabbit pAb (A12536)
ABclonal:Immunoprecipitation - [KO Validated] IKKγ Rabbit pAb (A12536)

Overview

Product name[KO Validated] IKKγ Rabbit pAb
Catalog No.A12536
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes the regulatory subunit of the inhibitor of kappaB kinase (IKK) complex, which activates NF-kappaB resulting in activation of genes involved in inflammation, immunity, cell survival, and other pathways. Mutations in this gene result in incontinentia pigmenti, hypohidrotic ectodermal dysplasia, and several other types of immunodeficiencies. A pseudogene highly similar to this locus is located in an adjacent region of the X chromosome.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-419 of human IKKγ (NP_003630.1).
SequenceMNRHLWKSQLCEMVQPSGGPAADQDVLGEESPLGKPAMLHLPSEQGAPETLQRCLEENQELRDAIRQSNQILRERCEELLHFQASQREEKEFLMCKFQEARKLVERLGLEKLDLKRQKEQALREVEHLKRCQQQMAEDKASVKAQVTSLLGELQESQSRLEAATKECQALEGRARAASEQARQLESEREALQQQHSVQVDQLRMQGQSVEAALRMERQAASEEKRKLAQLQVAYHQLFQEYDNHIKSSVVGSERKRGMQLEDLKQQLQQAEEALVAKQEVIDKLKEEAEQHKIVMETVPVLKAQADIYKADFQAERQAREKLAEKKELLQEQLEQLQREYSKLKASCQESARIEDMRKRHVEVSQAPLPPAPAYLSSPLALPSQRRSPPEEPPDFCCPKCQYQAPDMDTLQIHVMECIE
Gene ID8517
Swiss protQ9Y6K9
SynonymsIKBKG; AMCBX1; FIP-3; FIP3; Fip3p; IKK-gamma; IKKAP1; IKKG; IMD33; IP; IP1; IP2; IPD2; NEMO; ZC2HC9
Calculated MW36kDa/48kDa/55kDa
Observed MW48kDa
ReactivityHuman, Mouse
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
IP 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunoprecipitation    
Positive samplesA-549, Mouse liver
Cellular locationCytoplasm, Nucleus
ABclonal:Western blot - [KO Validated] IKKγ Rabbit pAb (A12536) }

Western blot - [KO Validated] IKKγ Rabbit pAb (A12536)

Western blot analysis of extracts of various cell lines, using IKKγ Antibody (A12536) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
ABclonal:Western blot - [KO Validated] IKKγ Rabbit pAb (A12536) }

Western blot - [KO Validated] IKKγ Rabbit pAb (A12536)

Western blot analysis of extracts from normal (control) and IKKγ knockout (KO) 293T cells, using IKKγ antibody (A12536) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.
ABclonal:Immunoprecipitation - [KO Validated] IKKγ Rabbit pAb (A12536) }

Immunoprecipitation - [KO Validated] IKKγ Rabbit pAb (A12536)

Immunoprecipitation analysis of 200ug extracts of A549 cells using 3ug IKKγ antibody (A12536). Western blot was performed from the immunoprecipitate using IKKγ antibody (A12536) at a dilition of 1:1000.

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