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[KO Validated] IFITM3 Rabbit pAb (A13070)

Publication (1) Datasheet

ABclonal:Western blot - [KO Validated] IFITM3 Rabbit pAb (A13070)
ABclonal:Western blot - [KO Validated] IFITM3 Rabbit pAb (A13070)
ABclonal:Immunohistochemistry - [KO Validated] IFITM3 Rabbit pAb (A13070)
ABclonal:Immunofluorescence - [KO Validated] IFITM3 Rabbit pAb (A13070)
ABclonal:Immunofluorescence - [KO Validated] IFITM3 Rabbit pAb (A13070)
ABclonal:Immunofluorescence - [KO Validated] IFITM3 Rabbit pAb (A13070)

Overview

Product name[KO Validated] IFITM3 Rabbit pAb
Catalog No.A13070
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is an interferon-induced membrane protein that helps confer immunity to influenza A H1N1 virus, West Nile virus, and dengue virus. Two transcript variants, only one of them protein-coding, have been found for this gene. Another variant encoding an N-terminally truncated isoform has been reported, but the full-length nature of this variant has not been determined.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-133 of human IFITM3 (NP_066362.2).
SequenceMNHTVQTFFSPVNSGQPPNYEMLKEEHEVAVLGAPHNPAPPTSTVIHIRSETSVPDHVVWSLFNTLFMNPCCLGFIAFAYSVKSRDRKMVGDVTGAQAYASTAKCLNIWALILGILMTILLIVIPVLIFQAYG
Gene ID10410
Swiss protQ01628
SynonymsIFITM3; 1-8U; DSPA2b; IP15
Calculated MW14kDa
Observed MW15kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesHeLa
Cellular locationCell membrane, Late endosome membrane, Lysosome membrane, Single-pass type II membrane protein
Customer validation

WB(Homo sapiens)

ABclonal:Western blot - [KO Validated] IFITM3 Rabbit pAb (A13070) }

Western blot - [KO Validated] IFITM3 Rabbit pAb (A13070)

Western blot analysis of extracts of HeLa cells, using IFITM3 antibody (A13070) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Western blot - [KO Validated] IFITM3 Rabbit pAb (A13070) }

Western blot - [KO Validated] IFITM3 Rabbit pAb (A13070)

Western blot analysis of extracts from normal (control) and IFITM3 knockout (KO) HeLa cells, using IFITM3 antibody (A13070) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Immunohistochemistry - [KO Validated] IFITM3 Rabbit pAb (A13070) }

Immunohistochemistry - [KO Validated] IFITM3 Rabbit pAb (A13070)

Immunohistochemistry of paraffin-embedded Human colon carcinoma using [KO Validated] IFITM3 Rabbit pAb (A13070) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - [KO Validated] IFITM3 Rabbit pAb (A13070) }

Immunofluorescence - [KO Validated] IFITM3 Rabbit pAb (A13070)

Immunofluorescence analysis of C6 cells using [KO] IFITM3 antibody (A13070) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - [KO Validated] IFITM3 Rabbit pAb (A13070) }

Immunofluorescence - [KO Validated] IFITM3 Rabbit pAb (A13070)

Immunofluorescence analysis of L929 cells using [KO] IFITM3 antibody (A13070) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - [KO Validated] IFITM3 Rabbit pAb (A13070) }

Immunofluorescence - [KO Validated] IFITM3 Rabbit pAb (A13070)

Immunofluorescence analysis of U-2 OS cells using [KO] IFITM3 antibody (A13070) at dilution of 1:100. Blue: DAPI for nuclear staining.

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