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[KO Validated] IDE Rabbit pAb (A1630)

Publication (1) Datasheet

ABclonal:Western blot - [KO Validated] IDE Polyclonal Antibody (A1630)
ABclonal:Western blot - [KO Validated] IDE Polyclonal Antibody (A1630)
ABclonal:Immunofluorescence - [KO Validated] IDE Polyclonal Antibody (A1630)

Overview

Product name[KO Validated] IDE Rabbit pAb
Catalog No.A1630
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a zinc metallopeptidase that degrades intracellular insulin, and thereby terminates insulins activity, as well as participating in intercellular peptide signalling by degrading diverse peptides such as glucagon, amylin, bradykinin, and kallidin. The preferential affinity of this enzyme for insulin results in insulin-mediated inhibition of the degradation of other peptides such as beta-amyloid. Deficiencies in this protein's function are associated with Alzheimer's disease and type 2 diabetes mellitus but mutations in this gene have not been shown to be causitive for these diseases. This protein localizes primarily to the cytoplasm but in some cell types localizes to the extracellular space, cell membrane, peroxisome, and mitochondrion. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional transcript variants have been described but have not been experimentally verified.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human IDE (NP_004960.2).
SequenceMRYRLAWLLHPALPSTFRSVLGARLPPPERLCGFQKKTYSKMNNPAIKRIGNHITKSPEDKREYRGLELANGIKVLLISDPTTDKSSAALDVHIGSLSDPPNIAGLSHFCEHMLFLGTKKYPKENEYSQFLSEHAGSSNAFTSGEHTNYYFDVSHEHLEGALDRFAQFFLCPLFDESCKDREVNAVDSEHEKNVMNDAWRLFQLEKATGNPKHPFSKFGTGNKYTLETRPNQEGIDVRQELLKFHSAYYS
Gene ID3416
Swiss protP14735
SynonymsIDE; INSULYSIN
Calculated MW54kDa/117kDa
Observed MW118kDa
ReactivityHuman, Mouse
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
IF 1:10 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunofluorescence    
Positive samplesDU145, Mouse liver
Cellular locationCell membrane, Cytoplasm, Secreted
Customer validation

WB(Mus musculus)

ABclonal:Western blot - [KO Validated] IDE Polyclonal Antibody (A1630) }

Western blot - [KO Validated] IDE Polyclonal Antibody (A1630)

Western blot analysis of extracts of various cell lines, using IDE antibody (A1630) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
ABclonal:Western blot - [KO Validated] IDE Polyclonal Antibody (A1630) }

Western blot - [KO Validated] IDE Polyclonal Antibody (A1630)

Western blot analysis of extracts from normal (control) and IDE knockout (KO) HeLa cells, using IDE antibody (A1630) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunofluorescence - [KO Validated] IDE Polyclonal Antibody (A1630) }

Immunofluorescence - [KO Validated] IDE Polyclonal Antibody (A1630)

Immunofluorescence analysis of A549 cells using IDE antibody (A1630). Blue: DAPI for nuclear staining.

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