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[KO Validated] HSPB1 Rabbit pAb (A16332)

Datasheet

ABclonal:Western blot - [KO Validated] HSPB1 Rabbit pAb (A16332)
ABclonal:Western blot - [KO Validated] HSPB1 Rabbit pAb (A16332)
ABclonal:Immunohistochemistry - [KO Validated] HSPB1 Rabbit pAb (A16332)
ABclonal:Immunohistochemistry - [KO Validated] HSPB1 Rabbit pAb (A16332)
ABclonal:Immunohistochemistry - [KO Validated] HSPB1 Rabbit pAb (A16332)
ABclonal:Immunohistochemistry - [KO Validated] HSPB1 Rabbit pAb (A16332)
ABclonal:Immunofluorescence - [KO Validated] HSPB1 Rabbit pAb (A16332)

Overview

Product name[KO Validated] HSPB1 Rabbit pAb
Catalog No.A16332
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is induced by environmental stress and developmental changes. The encoded protein is involved in stress resistance and actin organization and translocates from the cytoplasm to the nucleus upon stress induction. Defects in this gene are a cause of Charcot-Marie-Tooth disease type 2F (CMT2F) and distal hereditary motor neuropathy (dHMN).
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-120 of human HSPB1 (NP_001531.1).
SequenceMTERRVPFSLLRGPSWDPFRDWYPHSRLFDQAFGLPRLPEEWSQWLGGSSWPGYVRPLPPAAIESPAVAAPAYSRALSRQLSSGVSEIRHTADRWRVSLDVNHFAPDELTVKTKDGVVEI
Gene ID3315
Swiss protP04792
SynonymsHSPB1; CMT2F; HEL-S-102; HMN2B; HS.76067; HSP27; HSP28; Hsp25; SRP27
Calculated MW22kDa
Observed MW27KDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesHeLa
Cellular locationCytoplasm, Nucleus, cytoskeleton, spindle
ABclonal:Western blot - [KO Validated] HSPB1 Rabbit pAb (A16332) }

Western blot - [KO Validated] HSPB1 Rabbit pAb (A16332)

Western blot analysis of extracts from normal (control) and HSPB1 knockout (KO) HeLa cells, using HSPB1 antibody (A16332) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.
ABclonal:Western blot - [KO Validated] HSPB1 Rabbit pAb (A16332) }

Western blot - [KO Validated] HSPB1 Rabbit pAb (A16332)

Western blot analysis of extracts of HeLa cells, using HSPB1 antibody (A16332) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
ABclonal:Immunohistochemistry - [KO Validated] HSPB1 Rabbit pAb (A16332) }

Immunohistochemistry - [KO Validated] HSPB1 Rabbit pAb (A16332)

Immunohistochemistry of paraffin-embedded human breast cancer using HSPB1 antibody (A16332) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - [KO Validated] HSPB1 Rabbit pAb (A16332) }

Immunohistochemistry - [KO Validated] HSPB1 Rabbit pAb (A16332)

Immunohistochemistry of paraffin-embedded rat ovary using HSPB1 antibody (A16332) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - [KO Validated] HSPB1 Rabbit pAb (A16332) }

Immunohistochemistry - [KO Validated] HSPB1 Rabbit pAb (A16332)

Immunohistochemistry of paraffin-embedded rat brain using HSPB1 antibody (A16332) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - [KO Validated] HSPB1 Rabbit pAb (A16332) }

Immunohistochemistry - [KO Validated] HSPB1 Rabbit pAb (A16332)

Immunohistochemistry of paraffin-embedded mouse kidney using HSPB1 antibody (A16332) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - [KO Validated] HSPB1 Rabbit pAb (A16332) }

Immunofluorescence - [KO Validated] HSPB1 Rabbit pAb (A16332)

Immunofluorescence analysis of HeLa cells using HSPB1 antibody (A16332) at dilution of 1:100. Blue: DAPI for nuclear staining.

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