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[KO Validated] HPRT1 Rabbit pAb (A5692)

Datasheet

ABclonal:Western blot - [KO Validated] HPRT1 Polyclonal Antibody (A5692)
ABclonal:Western blot - [KO Validated] HPRT1 Polyclonal Antibody (A5692)
ABclonal:Immunofluorescence - [KO Validated] HPRT1 Polyclonal Antibody (A5692)

Overview

Product name[KO Validated] HPRT1 Rabbit pAb
Catalog No.A5692
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a transferase, which catalyzes conversion of hypoxanthine to inosine monophosphate and guanine to guanosine monophosphate via transfer of the 5-phosphoribosyl group from 5-phosphoribosyl 1-pyrophosphate. This enzyme plays a central role in the generation of purine nucleotides through the purine salvage pathway. Mutations in this gene result in Lesch-Nyhan syndrome or gout.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-218 of human HPRT1 (NP_000185.1).
SequenceMATRSPGVVISDDEPGYDLDLFCIPNHYAEDLERVFIPHGLIMDRTERLARDVMKEMGGHHIVALCVLKGGYKFFADLLDYIKALNRNSDRSIPMTVDFIRLKSYCNDQSTGDIKVIGGDDLSTLTGKNVLIVEDIIDTGKTMQTLLSLVRQYNPKMVKVASLLVKRTPRSVGYKPDFVGFEIPDKFVVGYALDYNEYFRDLNHVCVISETGKAKYKA
Gene ID3251
Swiss protP00492
SynonymsHPRT1; HGPRT; HPRT
Calculated MW24kDa
Observed MW27kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
IF 1:10 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunofluorescence    
Positive samplesMCF-7, HL-60, SW480, 293T, HeLa, Mouse brain, Mouse liver
Cellular locationCytoplasm
    ABclonal:Western blot - [KO Validated] HPRT1 Polyclonal Antibody (A5692) }

    Western blot - [KO Validated] HPRT1 Polyclonal Antibody (A5692)

    Western blot analysis of extracts of various cell lines, using HPRT1 antibody (A5692) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25ug per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 60s.
    ABclonal:Western blot - [KO Validated] HPRT1 Polyclonal Antibody (A5692) }

    Western blot - [KO Validated] HPRT1 Polyclonal Antibody (A5692)

    Western blot analysis of extracts from normal (control) and HPRT1 knockout (KO) 293T cells, using HPRT1 antibody (A5692) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25ug per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 5s.
    ABclonal:Immunofluorescence - [KO Validated] HPRT1 Polyclonal Antibody (A5692) }

    Immunofluorescence - [KO Validated] HPRT1 Polyclonal Antibody (A5692)

    Immunofluorescence analysis of U2OS cells using HPRT1 antibody (A5692). Blue: DAPI for nuclear staining.

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