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[KO Validated] HNRNPA2B1 Rabbit pAb (A1162)

Review (1)Publication (1) Datasheet

ABclonal:Western blot - [KO Validated] HNRNPA2B1 Rabbit pAb (A1162)
ABclonal:Western blot - [KO Validated] HNRNPA2B1 Rabbit pAb (A1162)
ABclonal:Immunohistochemistry - [KO Validated] HNRNPA2B1 Rabbit pAb (A1162)
ABclonal:Immunohistochemistry - [KO Validated] HNRNPA2B1 Rabbit pAb (A1162)
ABclonal:Immunohistochemistry - [KO Validated] HNRNPA2B1 Rabbit pAb (A1162)
ABclonal:Immunofluorescence - [KO Validated] HNRNPA2B1 Rabbit pAb (A1162)
ABclonal:Immunofluorescence - [KO Validated] HNRNPA2B1 Rabbit pAb (A1162)
Review
ABclonal: review for [KO Validated] HNRNPA2B1 Rabbit pAb(A1162)

Overview

Product name[KO Validated] HNRNPA2B1 Rabbit pAb
Catalog No.A1162
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene belongs to the A/B subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has two repeats of quasi-RRM domains that bind to RNAs. This gene has been described to generate two alternatively spliced transcript variants which encode different isoforms.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human HNRNPA2B1 (NP_002128.1).
SequenceMEREKEQFRKLFIGGLSFETTEESLRNYYEQWGKLTDCVVMRDPASKRSRGFGFVTFSSMAEVDAAMAARPHSIDGRVVEPKRAVAREESGKPGAHVTVKKLFVGGIKEDTEEHHLRDYFEEYGKIDTIEIITDRQSGKKRGFGFVTFDDHDPVDKIVLQKYHTINGHNAEVRKALSRQE
Gene ID3181
Swiss protP22626
SynonymsHNRNPA2; HNRNPB1; HNRPA2; HNRPA2B1; HNRPB1; IBMPFD2; RNPA2; SNRPB1; HNRNPA2B1
Calculated MW36kDa/37kDa
Observed MW37kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesHeLa, BxPC-3, PC-3, Mouse spleen
Cellular locationCytoplasm, Nucleus, nucleoplasm
Customer validation

(293T)

WB(Homo sapiens)

ABclonal:Western blot - [KO Validated] HNRNPA2B1 Rabbit pAb (A1162) }

Western blot - [KO Validated] HNRNPA2B1 Rabbit pAb (A1162)

Western blot analysis of extracts of various cell lines, using HNRNPA2B1 antibody (A1162) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
ABclonal:Western blot - [KO Validated] HNRNPA2B1 Rabbit pAb (A1162) }

Western blot - [KO Validated] HNRNPA2B1 Rabbit pAb (A1162)

Western blot analysis of extracts from normal (control) and HNRNPA2B1 knockout (KO) 293T cells, using HNRNPA2B1 antibody (A1162) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Immunohistochemistry - [KO Validated] HNRNPA2B1 Rabbit pAb (A1162) }

Immunohistochemistry - [KO Validated] HNRNPA2B1 Rabbit pAb (A1162)

Immunohistochemistry of paraffin-embedded mouse kidney using HNRNPA2B1 Antibody (A1162) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - [KO Validated] HNRNPA2B1 Rabbit pAb (A1162) }

Immunohistochemistry - [KO Validated] HNRNPA2B1 Rabbit pAb (A1162)

Immunohistochemistry of paraffin-embedded rat brain using HNRNPA2B1 Antibody (A1162) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - [KO Validated] HNRNPA2B1 Rabbit pAb (A1162) }

Immunohistochemistry - [KO Validated] HNRNPA2B1 Rabbit pAb (A1162)

Immunohistochemistry of paraffin-embedded human colon using HNRNPA2B1 Antibody (A1162) at dilution of 1:200 (40x lens).
ABclonal:Immunofluorescence - [KO Validated] HNRNPA2B1 Rabbit pAb (A1162) }

Immunofluorescence - [KO Validated] HNRNPA2B1 Rabbit pAb (A1162)

Immunofluorescence analysis of HeLa cells using HNRNPA2B1 antibody (A1162). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - [KO Validated] HNRNPA2B1 Rabbit pAb (A1162) }

Immunofluorescence - [KO Validated] HNRNPA2B1 Rabbit pAb (A1162)

Confocal immunofluorescence analysis of HeLa cells using HNRNPA2B1 Polyclonal Antibody (A1162) at dilution of 1:400. Blue: DAPI for nuclear staining.

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