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[KO Validated] HMGB1 Rabbit mAb (A19529)

Publication (1) Datasheet

ABclonal:[KO Validated] HMGB1 Rabbit mAb
ABclonal:Western blot - [KO Validated] HMGB1 Rabbit mAb (A19529)

Western blot analysis of extracts of various cell lines, using HMGB1 antibody (A19529) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

ABclonal:Western blot - [KO Validated] HMGB1 Rabbit mAb (A19529)

Western blot analysis of extracts from normal (control) and HMGB1 knockout (KO) 293T cells, using HMGB1 antibody (A19529) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

ABclonal:Western blot - [KO Validated] HMGB1 Rabbit mAb (A19529)

Western blot analysis of extracts of various cell lines, using HMGB1 antibody (A19529) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunohistochemistry - [KO Validated] HMGB1 Rabbit mAb (A19529)

Immunohistochemistry of paraffin-embedded Rat brain using HMGB1 antibody (A19529) at dilution of 1:100 (40x lens).

ABclonal:Immunohistochemistry - [KO Validated] HMGB1 Rabbit mAb (A19529)

Immunohistochemistry of paraffin-embedded Human esophagus using HMGB1 antibody (A19529) at dilution of 1:100 (40x lens).

ABclonal:Immunohistochemistry - [KO Validated] HMGB1 Rabbit mAb (A19529)

Immunohistochemistry of paraffin-embedded Mouse kidney using HMGB1 antibody (A19529) at dilution of 1:100 (40x lens).

ABclonal:Immunofluorescence - [KO Validated] HMGB1 Rabbit mAb (A19529)

Immunofluorescence analysis of C6 cells using HMGB1 antibody (A19529) at dilution of 1:100. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - [KO Validated] HMGB1 Rabbit mAb (A19529)

Immunofluorescence analysis of NIH/3T3 cells using HMGB1 antibody (A19529) at dilution of 1:100. Blue: DAPI for nuclear staining.

Overview

Product name[KO Validated] HMGB1 Rabbit mAb
Catalog No.A19529
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a protein that belongs to the High Mobility Group-box superfamily. The encoded non-histone, nuclear DNA-binding protein regulates transcription, and is involved in organization of DNA. This protein plays a role in several cellular processes, including inflammation, cell differentiation and tumor cell migration. Multiple pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Sep 2015]
ImmunogenA synthesized peptide derived from human HMGB1.
SequenceEmail for sequence
Gene ID3146
Swiss protP09429
SynonymsHMG-1; HMG1; HMG3; SBP-1; HMGB1
Calculated MW25kDa
Observed MW25kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samples293T, HeLa, Jurkat, Mouse liver, Mouse brain, Mouse testis, Rat liver
Cellular locationCell membrane, Chromosome, Cytoplasm, Endoplasmic reticulum-Golgi intermediate compartment, Endosome, Extracellular side, Nucleus, Peripheral membrane protein, Secreted
Customer validation

IF(Rattus norvegicus)

ABclonal:Western blot - [KO Validated] HMGB1 Rabbit mAb (A19529) }

Western blot - [KO Validated] HMGB1 Rabbit mAb (A19529)

Western blot analysis of extracts of various cell lines, using HMGB1 antibody (A19529) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.
ABclonal:Western blot - [KO Validated] HMGB1 Rabbit mAb (A19529) }

Western blot - [KO Validated] HMGB1 Rabbit mAb (A19529)

Western blot analysis of extracts from normal (control) and HMGB1 knockout (KO) 293T cells, using HMGB1 antibody (A19529) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.
ABclonal:Western blot - [KO Validated] HMGB1 Rabbit mAb (A19529) }

Western blot - [KO Validated] HMGB1 Rabbit mAb (A19529)

Western blot analysis of extracts of various cell lines, using HMGB1 antibody (A19529) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Immunohistochemistry - [KO Validated] HMGB1 Rabbit mAb (A19529) }

Immunohistochemistry - [KO Validated] HMGB1 Rabbit mAb (A19529)

Immunohistochemistry of paraffin-embedded Rat brain using HMGB1 antibody (A19529) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - [KO Validated] HMGB1 Rabbit mAb (A19529) }

Immunohistochemistry - [KO Validated] HMGB1 Rabbit mAb (A19529)

Immunohistochemistry of paraffin-embedded Human esophagus using HMGB1 antibody (A19529) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - [KO Validated] HMGB1 Rabbit mAb (A19529) }

Immunohistochemistry - [KO Validated] HMGB1 Rabbit mAb (A19529)

Immunohistochemistry of paraffin-embedded Mouse kidney using HMGB1 antibody (A19529) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - [KO Validated] HMGB1 Rabbit mAb (A19529) }

Immunofluorescence - [KO Validated] HMGB1 Rabbit mAb (A19529)

Immunofluorescence analysis of C6 cells using HMGB1 antibody (A19529) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - [KO Validated] HMGB1 Rabbit mAb (A19529) }

Immunofluorescence - [KO Validated] HMGB1 Rabbit mAb (A19529)

Immunofluorescence analysis of NIH/3T3 cells using HMGB1 antibody (A19529) at dilution of 1:100. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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