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[KO Validated] HK2 Rabbit pAb (A0994)

Reviews (4)Publications (7) Datasheet

ABclonal:Western blot - [KO Validated] HK2 Rabbit pAb (A0994)

Western blot analysis of extracts of various cell lines, using HK2 antibody (A0994) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Western blot - [KO Validated] HK2 Rabbit pAb (A0994)

Western blot analysis of extracts from normal (control) and HK2 knockout (KO) HeLa cells, using HK2 antibody (A0994) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunohistochemistry - [KO Validated] HK2 Rabbit pAb (A0994)

Immunohistochemistry of paraffin-embedded human breast cancer using HK2 antibody (A0994) at dilution of 1:200 (40x lens).

ABclonal:Immunohistochemistry - [KO Validated] HK2 Rabbit pAb (A0994)

Immunohistochemistry of paraffin-embedded human colon using HK2 antibody (A0994) at dilution of 1:200 (40x lens).

ABclonal:Immunohistochemistry - [KO Validated] HK2 Rabbit pAb (A0994)

Immunohistochemistry of paraffin-embedded mouse heart using HK2 antibody (A0994) at dilution of 1:200 (40x lens).

ABclonal:Immunohistochemistry - [KO Validated] HK2 Rabbit pAb (A0994)

Immunohistochemistry of paraffin-embedded rat heart using HK2 antibody (A0994) at dilution of 1:200 (40x lens).

ABclonal:Immunofluorescence - [KO Validated] HK2 Rabbit pAb (A0994)

Immunofluorescence analysis of NIH-3T3 cells using [KO Validated] HK2 Rabbit pAb (A0994) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - [KO Validated] HK2 Rabbit pAb (A0994)

Immunofluorescence analysis of PC-12 cells using [KO Validated] HK2 Rabbit pAb (A0994) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - [KO Validated] HK2 Rabbit pAb (A0994)

Immunofluorescence analysis of U-2 OS cells using [KO Validated] HK2 Rabbit pAb (A0994) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Review
ABclonal: review for [KO Validated] HK2 Rabbit pAb(A0994)
Review
ABclonal: review for [KO Validated] HK2 Rabbit pAb(A0994)
Review
ABclonal: review for [KO Validated] HK2 Rabbit pAb(A0994)
Review
ABclonal: review for [KO Validated] HK2 Rabbit pAb(A0994)

Overview

Product name[KO Validated] HK2 Rabbit pAb
Catalog No.A0994
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes hexokinase 2, the predominant form found in skeletal muscle. It localizes to the outer membrane of mitochondria. Expression of this gene is insulin-responsive, and studies in rat suggest that it is involved in the increased rate of glycolysis seen in rapidly growing cancer cells.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-120 of human HK2 (NP_000180.2).
SequenceMIASHLLAYFFTELNHDQVQKVDQYLYHMRLSDETLLEISKRFRKEMEKGLGATTHPTAAVKMLPTFVRSTPDGTEHGEFLALDLGGTNFRVLWVKVTDNGLQKVEMENQIYAIPEDIMR
Gene ID3099
Swiss protP52789
SynonymsHK2; HKII; HXK2; hexokinase-2
Calculated MW102kDa
Observed MW102KDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesSH-SY5Y, HeLa
Cellular locationMitochondrion outer membrane
Customer validation

(H460, unknow, SK-N-SH, U251,U87MG)

WB(Rattus norvegicus, Homo sapiens, Mus musculus)

IHC(Homo sapiens,Mus musculus)

ABclonal:Western blot - [KO Validated] HK2 Rabbit pAb (A0994) }

Western blot - [KO Validated] HK2 Rabbit pAb (A0994)

Western blot analysis of extracts of various cell lines, using HK2 antibody (A0994) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Western blot - [KO Validated] HK2 Rabbit pAb (A0994) }

Western blot - [KO Validated] HK2 Rabbit pAb (A0994)

Western blot analysis of extracts from normal (control) and HK2 knockout (KO) HeLa cells, using HK2 antibody (A0994) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunohistochemistry - [KO Validated] HK2 Rabbit pAb (A0994) }

Immunohistochemistry - [KO Validated] HK2 Rabbit pAb (A0994)

Immunohistochemistry of paraffin-embedded human breast cancer using HK2 antibody (A0994) at dilution of 1:200 (40x lens).
ABclonal:Immunohistochemistry - [KO Validated] HK2 Rabbit pAb (A0994) }

Immunohistochemistry - [KO Validated] HK2 Rabbit pAb (A0994)

Immunohistochemistry of paraffin-embedded human colon using HK2 antibody (A0994) at dilution of 1:200 (40x lens).
ABclonal:Immunohistochemistry - [KO Validated] HK2 Rabbit pAb (A0994) }

Immunohistochemistry - [KO Validated] HK2 Rabbit pAb (A0994)

Immunohistochemistry of paraffin-embedded mouse heart using HK2 antibody (A0994) at dilution of 1:200 (40x lens).
ABclonal:Immunohistochemistry - [KO Validated] HK2 Rabbit pAb (A0994) }

Immunohistochemistry - [KO Validated] HK2 Rabbit pAb (A0994)

Immunohistochemistry of paraffin-embedded rat heart using HK2 antibody (A0994) at dilution of 1:200 (40x lens).
ABclonal:Immunofluorescence - [KO Validated] HK2 Rabbit pAb (A0994) }

Immunofluorescence - [KO Validated] HK2 Rabbit pAb (A0994)

Immunofluorescence analysis of NIH-3T3 cells using [KO Validated] HK2 Rabbit pAb (A0994) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - [KO Validated] HK2 Rabbit pAb (A0994) }

Immunofluorescence - [KO Validated] HK2 Rabbit pAb (A0994)

Immunofluorescence analysis of PC-12 cells using [KO Validated] HK2 Rabbit pAb (A0994) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - [KO Validated] HK2 Rabbit pAb (A0994) }

Immunofluorescence - [KO Validated] HK2 Rabbit pAb (A0994)

Immunofluorescence analysis of U-2 OS cells using [KO Validated] HK2 Rabbit pAb (A0994) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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