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[KO Validated] HINT1 Rabbit pAb (A10221)

Datasheet

ABclonal:Western blot - [KO Validated] HINT1 Rabbit pAb (A10221)
ABclonal:Western blot - [KO Validated] HINT1 Rabbit pAb (A10221)
ABclonal:Immunofluorescence - [KO Validated] HINT1 Rabbit pAb (A10221)
ABclonal:Immunofluorescence - [KO Validated] HINT1 Rabbit pAb (A10221)

Overview

Product name[KO Validated] HINT1 Rabbit pAb
Catalog No.A10221
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a protein that hydrolyzes purine nucleotide phosphoramidates substrates, including AMP-morpholidate, AMP-N-alanine methyl ester, AMP-alpha-acetyl lysine methyl ester, and AMP-NH2. The encoded protein interacts with these substrates via a histidine triad motif. This gene is considered a tumor suppressor gene. In addition, mutations in this gene can cause autosomal recessive neuromyotonia and axonal neuropathy. There are several related pseudogenes on chromosome 7. Several transcript variants have been observed.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-126 of human HINT1 (NP_005331.1).
SequenceMADEIAKAQVARPGGDTIFGKIIRKEIPAKIIFEDDRCLAFHDISPQAPTHFLVIPKKHISQISVAEDDDESLLGHLMIVGKKCAADLGLNKGYRMVVNEGSDGGQSVYHVHLHVLGGRQMHWPPG
Gene ID3094
Swiss protP49773
SynonymsHINT1; HINT; NMAN; PKCI-1; PRKCNH1
Calculated MW13kDa
Observed MW14kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:1000 - 1:2000
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunofluorescence    
Positive samples22Rv1, HeLa, H460, K-562, Mouse kidney, Mouse liver, Rat kidney, Rat liver
Cellular locationCytoplasm, Nucleus
    ABclonal:Western blot - [KO Validated] HINT1 Rabbit pAb (A10221) }

    Western blot - [KO Validated] HINT1 Rabbit pAb (A10221)

    Western blot analysis of extracts of various cell lines, using HINT1 antibody (A10221) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25ug per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 1s.
    ABclonal:Western blot - [KO Validated] HINT1 Rabbit pAb (A10221) }

    Western blot - [KO Validated] HINT1 Rabbit pAb (A10221)

    Western blot analysis of extracts from normal (control) and HINT1 knockout (KO) HeLa cells, using HINT1 antibody (A10221) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25ug per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 1s.
    ABclonal:Immunofluorescence - [KO Validated] HINT1 Rabbit pAb (A10221) }

    Immunofluorescence - [KO Validated] HINT1 Rabbit pAb (A10221)

    Immunofluorescence analysis of H9C2 cells using [KO Validated] HINT1 Rabbit pAb (A10221) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
    ABclonal:Immunofluorescence - [KO Validated] HINT1 Rabbit pAb (A10221) }

    Immunofluorescence - [KO Validated] HINT1 Rabbit pAb (A10221)

    Immunofluorescence analysis of L929 cells using [KO Validated] HINT1 Rabbit pAb (A10221) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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