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[KO Validated] HER2 / ErbB2 Rabbit pAb (A2071)

Publications (2) Datasheet

ABclonal:Western blot - [KO Validated] HER2 / ErbB2 Rabbit pAb (A2071)

Western blot analysis of extracts from normal (control) and HER2 / ErbB2 knockout (KO) HeLa cells, using HER2 / ErbB2 antibody (A2071) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5min.

ABclonal:Western blot - [KO Validated] HER2 / ErbB2 Rabbit pAb (A2071)

Western blot analysis of extracts of SK-BR-3 cells, using HER2 / ErbB2 antibody (A2071) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Western blot - [KO Validated] HER2 / ErbB2 Rabbit pAb (A2071)

Western blot analysis of extracts of Mouse lung, using HER2 / ErbB2 antibody (A2071) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Immunohistochemistry - [KO Validated] HER2 / ErbB2 Rabbit pAb (A2071)

Immunohistochemistry of paraffin-embedded rat testis using [KO Validated] HER2 / ErbB2 Rabbit pAb (A2071) at dilution of 1:100 (40x lens).

Overview

Product name[KO Validated] HER2 / ErbB2 Rabbit pAb
Catalog No.A2071
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Allelic variations at amino acid positions 654 and 655 of isoform a (positions 624 and 625 of isoform b) have been reported, with the most common allele, Ile654/Ile655, shown here. Amplification and/or overexpression of this gene has been reported in numerous cancers, including breast and ovarian tumors. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1160 to the C-terminus of human HER2 / ErbB2 (NP_004439.2).
SequenceARPAGATLERPKTLSPGKNGVVKDVFAFGGAVENPEYLTPQGGAAPQPHPPPAFSPAFDNLYYWDQDPPERGAPPSTFKGTPTAENPEYLGLDVPV
Gene ID2064
Swiss protP04626
SynonymsERBB2; CD340; HER-2; HER-2/neu; HER2; MLN 19; NEU; NGL; TKR1; erb-b2 receptor tyrosine kinase 2; HER2/ErbB2; ErbB2; MLN19
Calculated MW62kDa/70kDa/97kDa/134kDa/136kDa/137kDa
Observed MW185KDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    
Positive samplesSK-BR-3, Mouse lung
Cellular locationCell membrane, Cytoplasm, Nucleus, Nucleus, Single-pass type I membrane protein, perinuclear region
Customer validation

WB(Mus musculus, Homo sapiens)

ABclonal:Western blot - [KO Validated] HER2 / ErbB2 Rabbit pAb (A2071) }

Western blot - [KO Validated] HER2 / ErbB2 Rabbit pAb (A2071)

Western blot analysis of extracts from normal (control) and HER2 / ErbB2 knockout (KO) HeLa cells, using HER2 / ErbB2 antibody (A2071) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5min.
ABclonal:Western blot - [KO Validated] HER2 / ErbB2 Rabbit pAb (A2071) }

Western blot - [KO Validated] HER2 / ErbB2 Rabbit pAb (A2071)

Western blot analysis of extracts of SK-BR-3 cells, using HER2 / ErbB2 antibody (A2071) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Western blot - [KO Validated] HER2 / ErbB2 Rabbit pAb (A2071) }

Western blot - [KO Validated] HER2 / ErbB2 Rabbit pAb (A2071)

Western blot analysis of extracts of Mouse lung, using HER2 / ErbB2 antibody (A2071) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
ABclonal:Immunohistochemistry - [KO Validated] HER2 / ErbB2 Rabbit pAb (A2071) }

Immunohistochemistry - [KO Validated] HER2 / ErbB2 Rabbit pAb (A2071)

Immunohistochemistry of paraffin-embedded rat testis using [KO Validated] HER2 / ErbB2 Rabbit pAb (A2071) at dilution of 1:100 (40x lens).

* For research use only. Not for therapeutic or diagnostic purposes.

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