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[KO Validated] HELLS Rabbit pAb (A5831)

Publications (2) Datasheet

ABclonal:Western blot - [KO Validated] HELLS Polyclonal Antibody (A5831)
ABclonal:Western blot - [KO Validated] HELLS Polyclonal Antibody (A5831)
ABclonal:Immunohistochemistry - [KO Validated] HELLS Polyclonal Antibody (A5831)
ABclonal:Immunoprecipitation - [KO Validated] HELLS Polyclonal Antibody (A5831)

Overview

Product name[KO Validated] HELLS Rabbit pAb
Catalog No.A5831
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a lymphoid-specific helicase. Other helicases function in processes involving DNA strand separation, including replication, repair, recombination, and transcription. This protein is thought to be involved with cellular proliferation and may play a role in leukemogenesis. Alternatively spliced transcript variants encoding different isoforms have been identified.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 719-838 of human HELLS (NP_060533.2).
SequenceVYRLVTANTIDQKIVERAAAKRKLEKLIIHKNHFKGGQSGLNLSKNFLDPKELMELLKSRDYEREIKGSREKVISDKDLELLLDRSDLIDQMNASGPIKEKMGIFKILENSEDSSPECLF
Gene ID3070
Swiss protQ9NRZ9
SynonymsHELLS; ICF4; LSH; Nbla10143; PASG; SMARCA6
Calculated MW14kDa/36-39kDa/81-97kDa
Observed MW120kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IP 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunoprecipitation    
Positive samplesMCF-7, A549, 293T, HepG2, Mouse thymus
Cellular locationNucleus
Customer validation

WB(Homo sapiens)

ABclonal:Western blot - [KO Validated] HELLS Polyclonal Antibody (A5831) }

Western blot - [KO Validated] HELLS Polyclonal Antibody (A5831)

Western blot analysis of extracts of various cell lines, using HELLS antibody (A5831) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Western blot - [KO Validated] HELLS Polyclonal Antibody (A5831) }

Western blot - [KO Validated] HELLS Polyclonal Antibody (A5831)

Western blot analysis of extracts from normal (control) and HELLS knockout (KO) HeLa cells, using HELLS antibody (A5831) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.
ABclonal:Immunohistochemistry - [KO Validated] HELLS Polyclonal Antibody (A5831) }

Immunohistochemistry - [KO Validated] HELLS Polyclonal Antibody (A5831)

Immunohistochemistry of paraffin-embedded mouse testis using HELLS Antibody (A5831) at dilution of 1:100 (40x lens).
ABclonal:Immunoprecipitation - [KO Validated] HELLS Polyclonal Antibody (A5831) }

Immunoprecipitation - [KO Validated] HELLS Polyclonal Antibody (A5831)

Immunoprecipitation analysis of 200ug extracts of 293T cells using 1ug HELLS antibody (A5831). Western blot was performed from the immunoprecipitate using HELLS antibody (A5831) at a dilition of 1:1000.

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