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[KO Validated] HDAC8 Rabbit pAb (A5829)

Publication (1) Datasheet

ABclonal:Western blot - [KO Validated] HDAC8 Polyclonal Antibody (A5829)
ABclonal:Western blot - [KO Validated] HDAC8 Polyclonal Antibody (A5829)
ABclonal:Immunohistochemistry - [KO Validated] HDAC8 Polyclonal Antibody (A5829)
ABclonal:Immunohistochemistry - [KO Validated] HDAC8 Polyclonal Antibody (A5829)
ABclonal:Immunohistochemistry - [KO Validated] HDAC8 Polyclonal Antibody (A5829)

Overview

Product name[KO Validated] HDAC8 Rabbit pAb
Catalog No.A5829
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to class I of the histone deacetylase family. It catalyzes the deacetylation of lysine residues in the histone N-terminal tails and represses transcription in large multiprotein complexes with transcriptional co-repressors. Multiple transcript variants encoding different isoforms have been found for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-377 of human HDAC8 (NP_060956.1).
SequenceMEEPEEPADSGQSLVPVYIYSPEYVSMCDSLAKIPKRASMVHSLIEAYALHKQMRIVKPKVASMEEMATFHTDAYLQHLQKVSQEGDDDHPDSIEYGLGYDCPATEGIFDYAAAIGGATITAAQCLIDGMCKVAINWSGGWHHAKKDEASGFCYLNDAVLGILRLRRKFERILYVDLDLHHGDGVEDAFSFTSKVMTVSLHKFSPGFFPGTGDVSDVGLGKGRYYSVNVPIQDGIQDEKYYQICESVLKEVYQAFNPKAVVLQLGADTIAGDPMCSFNMTPVGIGKCLKYILQWQLATLILGGGGYNLANTARCWTYLTGVILGKTLSSEIPDHEFFTAYGPDYVLEITPSCRPDRNEPHRIQQILNYIKGNLKHVV
Gene ID55869
Swiss protQ9BY41
SynonymsHDAC8; CDA07; CDLS5; HD8; HDACL1; MRXS6; RPD3; WTS
Calculated MW15kDa/16kDa/17kDa/28kDa/29kDa/31kDa/41kDa
Observed MW42kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samples293T, Mouse lung
Cellular locationCytoplasm, Nucleus
Customer validation

WB(Homo sapiens)

ABclonal:Western blot - [KO Validated] HDAC8 Polyclonal Antibody (A5829) }

Western blot - [KO Validated] HDAC8 Polyclonal Antibody (A5829)

Western blot analysis of extracts of various cell lines, using HDAC8 Antibody (A5829) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Western blot - [KO Validated] HDAC8 Polyclonal Antibody (A5829) }

Western blot - [KO Validated] HDAC8 Polyclonal Antibody (A5829)

Western blot analysis of extracts from normal (control) and HDAC8 knockout (KO) HeLa cells, using HDAC8 antibody (A5829) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Immunohistochemistry - [KO Validated] HDAC8 Polyclonal Antibody (A5829) }

Immunohistochemistry - [KO Validated] HDAC8 Polyclonal Antibody (A5829)

Immunohistochemistry of paraffin-embedded rat testis using HDAC8 antibody (A5829) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - [KO Validated] HDAC8 Polyclonal Antibody (A5829) }

Immunohistochemistry - [KO Validated] HDAC8 Polyclonal Antibody (A5829)

Immunohistochemistry of paraffin-embedded rat spinal cord using HDAC8 antibody (A5829) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - [KO Validated] HDAC8 Polyclonal Antibody (A5829) }

Immunohistochemistry - [KO Validated] HDAC8 Polyclonal Antibody (A5829)

Immunohistochemistry of paraffin-embedded human breast cancer using HDAC8 antibody (A5829) at dilution of 1:100 (40x lens).

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