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[KO Validated] HADHA Rabbit pAb (A5346)

Review (1) Datasheet

ABclonal:Western blot - [KO Validated] HADHA Polyclonal Antibody (A5346)
ABclonal:Western blot - [KO Validated] HADHA Polyclonal Antibody (A5346)
ABclonal:Immunofluorescence - [KO Validated] HADHA Polyclonal Antibody (A5346)
ABclonal:Immunoprecipitation - [KO Validated] HADHA Polyclonal Antibody (A5346)
Review
ABclonal: review for [KO Validated] HADHA Rabbit pAb(A5346)

Overview

Product name[KO Validated] HADHA Rabbit pAb
Catalog No.A5346
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes the alpha subunit of the mitochondrial trifunctional protein, which catalyzes the last three steps of mitochondrial beta-oxidation of long chain fatty acids. The mitochondrial membrane-bound heterocomplex is composed of four alpha and four beta subunits, with the alpha subunit catalyzing the 3-hydroxyacyl-CoA dehydrogenase and enoyl-CoA hydratase activities. Mutations in this gene result in trifunctional protein deficiency or LCHAD deficiency. The genes of the alpha and beta subunits of the mitochondrial trifunctional protein are located adjacent to each other in the human genome in a head-to-head orientation.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 484-763 of human HADHA (NP_000173.2).
SequenceIAAVSKRPEKVIGMHYFSPVDKMQLLEIITTEKTSKDTSASAVAVGLKQGKVIIVVKDGPGFYTTRCLAPMMSEVIRILQEGVDPKKLDSLTTSFGFPVGAATLVDEVGVDVAKHVAEDLGKVFGERFGGGNPELLTQMVSKGFLGRKSGKGFYIYQEGVKRKDLNSDMDSILASLKLPPKSEVSSDEDIQFRLVTRFVNEAVMCLQEGILATPAEGDIGAVFGLGFPPCLGGPFRFVDLYGAQKIVDRLKKYEAAYGKQFTPCQLLADHANSPNKKFYQ
Gene ID3030
Swiss protP40939
SynonymsHADHA; ECHA; GBP; HADH; LCEH; LCHAD; MTPA; TP-ALPHA
Calculated MW28kDa/83kDa
Observed MW82kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
IF 1:50 - 1:200
IP 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunofluorescence    Immunoprecipitation    
Positive samplesK-562, THP-1, SW480, PC-3, HeLa, Mouse intestine, Mouse heart, Mouse adipose
Cellular locationMitochondrion
Customer validation

(293T)

ABclonal:Western blot - [KO Validated] HADHA Polyclonal Antibody (A5346) }

Western blot - [KO Validated] HADHA Polyclonal Antibody (A5346)

Western blot analysis of extracts of various cell lines, using HADHA antibody (A5346) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.
ABclonal:Western blot - [KO Validated] HADHA Polyclonal Antibody (A5346) }

Western blot - [KO Validated] HADHA Polyclonal Antibody (A5346)

Western blot analysis of extracts from normal (control) and HADHA knockout (KO) 293T cells, using HADHA antibody (A5346) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Immunofluorescence - [KO Validated] HADHA Polyclonal Antibody (A5346) }

Immunofluorescence - [KO Validated] HADHA Polyclonal Antibody (A5346)

Immunofluorescence analysis of U2OS cells using HADHA antibody (A5346). Blue: DAPI for nuclear staining.
ABclonal:Immunoprecipitation - [KO Validated] HADHA Polyclonal Antibody (A5346) }

Immunoprecipitation - [KO Validated] HADHA Polyclonal Antibody (A5346)

Immunoprecipitation analysis of 200ug extracts of HeLa cells using 3ug HADHA antibody (A5346). Western blot was performed from the immunoprecipitate using HADHA antibody (A5346) at a dilition of 1:1000.

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