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[KO Validated] GSS Rabbit pAb (A14535)

Datasheet

ABclonal:Western blot - [KO Validated] GSS Polyclonal Antibody (A14535)
ABclonal:Western blot - [KO Validated] GSS Polyclonal Antibody (A14535)

Overview

Product name[KO Validated] GSS Rabbit pAb
Catalog No.A14535
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Glutathione is important for a variety of biological functions, including protection of cells from oxidative damage by free radicals, detoxification of xenobiotics, and membrane transport. The protein encoded by this gene functions as a homodimer to catalyze the second step of glutathione biosynthesis, which is the ATP-dependent conversion of gamma-L-glutamyl-L-cysteine to glutathione. Defects in this gene are a cause of glutathione synthetase deficiency.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 373-474 of human GSS (NP_000169.1).
SequenceNLYGEEMVQALKQLKDSEERASYILMEKIEPEPFENCLLRPGSPARVVQCISELGIFGVYVRQEKTLVMNKHVGHLLRTKAIEHADGGVAAGVAVLDNPYPV
Gene ID2937
Swiss protP48637
SynonymsGSS; GSHS; HEL-S-64p; HEL-S-88n
Calculated MW40kDa/52kDa
Observed MW54kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    
Positive samplesDU145, K-562, 293T, Mouse kidney, Mouse spleen, Mouse brain, Rat kidney
Cellular location
ABclonal:Western blot - [KO Validated] GSS Polyclonal Antibody (A14535) }

Western blot - [KO Validated] GSS Polyclonal Antibody (A14535)

Western blot analysis of extracts of various cell lines, using GSS antibody (A14535) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.
ABclonal:Western blot - [KO Validated] GSS Polyclonal Antibody (A14535) }

Western blot - [KO Validated] GSS Polyclonal Antibody (A14535)

Western blot analysis of extracts from normal (control) and GSS knockout (KO) 293T cells, using GSS antibody (A14535) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

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