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[KO Validated] GNAI3 Rabbit pAb (A13307)

Datasheet

ABclonal:Western blot - [KO Validated] GNAI3 Rabbit pAb (A13307)
ABclonal:Western blot - [KO Validated] GNAI3 Rabbit pAb (A13307)
ABclonal:Immunofluorescence - [KO Validated] GNAI3 Rabbit pAb (A13307)
ABclonal:Immunoprecipitation - [KO Validated] GNAI3 Rabbit pAb (A13307)

Overview

Product name[KO Validated] GNAI3 Rabbit pAb
Catalog No.A13307
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling pathways. G proteins are composed of 3 units: alpha, beta and gamma. This gene encodes an alpha subunit and belongs to the G-alpha family. Mutation in this gene, resulting in a gly40-to-arg substitution, is associated with auriculocondylar syndrome, and shown to affect downstream targets in the G protein-coupled endothelin receptor pathway.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-354 of human GNAI3 (NP_006487.1).
SequenceMGCTLSAEDKAAVERSKMIDRNLREDGEKAAKEVKLLLLGAGESGKSTIVKQMKIIHEDGYSEDECKQYKVVVYSNTIQSIIAIIRAMGRLKIDFGEAARADDARQLFVLAGSAEEGVMTPELAGVIKRLWRDGGVQACFSRSREYQLNDSASYYLNDLDRISQSNYIPTQQDVLRTRVKTTGIVETHFTFKDLYFKMFDVGGQRSERKKWIHCFEGVTAIIFCVALSDYDLVLAEDEEMNRMHESMKLFDSICNNKWFTETSIILFLNKKDLFEEKIKRSPLTICYPEYTGSNTYEEAAAYIQCQFEDLNRRKDTKEIYTHFTCATDTKNVQFVFDAVTDVIIKNNLKECGLY
Gene ID2773
Swiss protP08754
SynonymsGNAI3; 87U6; ARCND1
Calculated MW40kDa
Observed MW45kDa
ReactivityHuman, Mouse
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
IF 1:50 - 1:200
IP 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunofluorescence    Immunoprecipitation    
Positive samplesLO2, MCF-7, HL-60, HT-1080
Cellular locationCell membrane, Cytoplasm, Lipid-anchor, Membrane, centrosome, cytoskeleton, microtubule organizing center
ABclonal:Western blot - [KO Validated] GNAI3 Rabbit pAb (A13307) }

Western blot - [KO Validated] GNAI3 Rabbit pAb (A13307)

Western blot analysis of extracts of various cell lines, using GNAI3 antibody (A13307) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Western blot - [KO Validated] GNAI3 Rabbit pAb (A13307) }

Western blot - [KO Validated] GNAI3 Rabbit pAb (A13307)

Western blot analysis of extracts from normal (control) and GNAI3 knockout (KO) HeLa cells, using GNAI3 antibody (A13307) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Immunofluorescence - [KO Validated] GNAI3 Rabbit pAb (A13307) }

Immunofluorescence - [KO Validated] GNAI3 Rabbit pAb (A13307)

Immunofluorescence analysis of L929 cells using [KO Validated] GNAI3 Rabbit pAb (A13307) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunoprecipitation - [KO Validated] GNAI3 Rabbit pAb (A13307) }

Immunoprecipitation - [KO Validated] GNAI3 Rabbit pAb (A13307)

Immunoprecipitation analysis of 200ug extracts of MCF-7 cells, using 3 ug GNAI3 antibody (A13307). Western blot was performed from the immunoprecipitate using GNAI3 antibody (A13307) at a dilition of 1:1000.

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