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[KO Validated] GNA13 Rabbit pAb (A5746)

Datasheet

ABclonal:Western blot - [KO Validated] GNA13 Polyclonal Antibody (A5746)
ABclonal:Western blot - [KO Validated] GNA13 Polyclonal Antibody (A5746)
ABclonal:Immunohistochemistry - [KO Validated] GNA13 Polyclonal Antibody (A5746)
ABclonal:Immunohistochemistry - [KO Validated] GNA13 Polyclonal Antibody (A5746)
ABclonal:Immunofluorescence - [KO Validated] GNA13 Polyclonal Antibody (A5746)
ABclonal:Immunofluorescence - [KO Validated] GNA13 Polyclonal Antibody (A5746)

Overview

Product name[KO Validated] GNA13 Rabbit pAb
Catalog No.A5746
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
ImmunogenRecombinant protein of human GNA13
SequenceEmail for sequence
Gene ID10672
Swiss protQ14344
SynonymsGNA13; G13
Calculated MW33kDa/44kDa
Observed MW44kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
IHC 1:100 - 1:200
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samples293T, BT-474, Jurkat, Mouse kidney, Mouse lung, Mouse spleen, Rat kidney
Cellular locationCytoplasm, Lipid-anchor, Melanosome, Membrane, Nucleus
ABclonal:Western blot - [KO Validated] GNA13 Polyclonal Antibody (A5746) }

Western blot - [KO Validated] GNA13 Polyclonal Antibody (A5746)

Western blot analysis of extracts of various cell lines, using GNA13 antibody (A5746) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Western blot - [KO Validated] GNA13 Polyclonal Antibody (A5746) }

Western blot - [KO Validated] GNA13 Polyclonal Antibody (A5746)

Western blot analysis of extracts from normal (control) and GNA13 knockout (KO) HeLa cells, using GNA13 antibody (A5746) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunohistochemistry - [KO Validated] GNA13 Polyclonal Antibody (A5746) }

Immunohistochemistry - [KO Validated] GNA13 Polyclonal Antibody (A5746)

Immunohistochemistry of paraffin-embedded human stomach using GNA13 antibody (A5746) (40x lens).
ABclonal:Immunohistochemistry - [KO Validated] GNA13 Polyclonal Antibody (A5746) }

Immunohistochemistry - [KO Validated] GNA13 Polyclonal Antibody (A5746)

Immunohistochemistry of paraffin-embedded mouse kidney using GNA13 antibody (A5746) (40x lens).
ABclonal:Immunofluorescence - [KO Validated] GNA13 Polyclonal Antibody (A5746) }

Immunofluorescence - [KO Validated] GNA13 Polyclonal Antibody (A5746)

Immunofluorescence analysis of C6 cells using GNA13 Polyclonal Antibody (A5746) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - [KO Validated] GNA13 Polyclonal Antibody (A5746) }

Immunofluorescence - [KO Validated] GNA13 Polyclonal Antibody (A5746)

Immunofluorescence analysis of NIH-3T3 cells using GNA13 Polyclonal Antibody (A5746) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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