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[KO Validated] GNA13 Rabbit pAb (A5746)

Datasheet

ABclonal:Western blot - [KO Validated] GNA13 Polyclonal Antibody (A5746)

ABclonal:Immunohistochemistry - [KO Validated] GNA13 Polyclonal Antibody (A5746)

ABclonal:Immunofluorescence - [KO Validated] GNA13 Polyclonal Antibody (A5746)

Overview

Product name	[KO Validated] GNA13 Rabbit pAb
Catalog No.	A5746
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Immunogen information

Immunogen	Recombinant protein of human GNA13
Sequence	Email for sequence
Gene ID	10672
Swiss prot	Q14344
Synonyms	GNA13; G13
Calculated MW	33kDa/44kDa
Observed MW	44kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC ICC IF IP ChIP ChIPseq RIP FC ELISA MeDIP Nucleotide Array DB
Recommended dilution	WB 1:500 - 1:2000 IHC 1:100 - 1:200 IF 1:50 - 1:200
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key	Western blotting Immunohistochemistry Immunofluorescence
Positive samples	293T, BT-474, Jurkat, Mouse kidney, Mouse lung, Mouse spleen, Rat kidney
Cellular location	Cytoplasm, Lipid-anchor, Melanosome, Membrane, Nucleus

[KO Validated] GNA13 Rabbit pAb images

Western blot - [KO Validated] GNA13 Polyclonal Antibody (A5746)

Western blot analysis of extracts of various cell lines, using GNA13 antibody (A5746) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

Western blot - [KO Validated] GNA13 Polyclonal Antibody (A5746)

Western blot analysis of extracts from normal (control) and GNA13 knockout (KO) HeLa cells, using GNA13 antibody (A5746) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

Immunohistochemistry - [KO Validated] GNA13 Polyclonal Antibody (A5746)

Immunohistochemistry of paraffin-embedded human stomach using GNA13 antibody (A5746) (40x lens).

Immunohistochemistry - [KO Validated] GNA13 Polyclonal Antibody (A5746)

Immunohistochemistry of paraffin-embedded mouse kidney using GNA13 antibody (A5746) (40x lens).

Immunofluorescence - [KO Validated] GNA13 Polyclonal Antibody (A5746)

Immunofluorescence analysis of C6 cells using GNA13 Polyclonal Antibody (A5746) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunofluorescence - [KO Validated] GNA13 Polyclonal Antibody (A5746)

Immunofluorescence analysis of NIH-3T3 cells using GNA13 Polyclonal Antibody (A5746) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Product Protocols

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