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[KO Validated] GLUL Rabbit pAb (A5437)

Review (1) Datasheet

ABclonal:Western blot - [KO Validated] GLUL Rabbit pAb (A5437)
ABclonal:Western blot - [KO Validated] GLUL Rabbit pAb (A5437)
ABclonal:Immunohistochemistry - [KO Validated] GLUL Rabbit pAb (A5437)
ABclonal:Immunohistochemistry - [KO Validated] GLUL Rabbit pAb (A5437)
ABclonal:Immunohistochemistry - [KO Validated] GLUL Rabbit pAb (A5437)
ABclonal:Immunofluorescence - [KO Validated] GLUL Rabbit pAb (A5437)
Review
ABclonal: review for [KO Validated] GLUL Rabbit pAb(A5437)

Overview

Product name[KO Validated] GLUL Rabbit pAb
Catalog No.A5437
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene belongs to the glutamine synthetase family. It catalyzes the synthesis of glutamine from glutamate and ammonia in an ATP-dependent reaction. This protein plays a role in ammonia and glutamate detoxification, acid-base homeostasis, cell signaling, and cell proliferation. Glutamine is an abundant amino acid, and is important to the biosynthesis of several amino acids, pyrimidines, and purines. Mutations in this gene are associated with congenital glutamine deficiency, and overexpression of this gene was observed in some primary liver cancer samples. There are six pseudogenes of this gene found on chromosomes 2, 5, 9, 11, and 12. Alternative splicing results in multiple transcript variants.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-373 of human GLUL (NP_001028228.1).
SequenceMTTSASSHLNKGIKQVYMSLPQGEKVQAMYIWIDGTGEGLRCKTRTLDSEPKCVEELPEWNFDGSSTLQSEGSNSDMYLVPAAMFRDPFRKDPNKLVLCEVFKYNRRPAETNLRHTCKRIMDMVSNQHPWFGMEQEYTLMGTDGHPFGWPSNGFPGPQGPYYCGVGADRAYGRDIVEAHYRACLYAGVKIAGTNAEVMPAQWEFQIGPCEGISMGDHLWVARFILHRVCEDFGVIATFDPKPIPGNWNGAGCHTNFSTKAMREENGLKYIEEAIEKLSKRHQYHIRAYDPKGGLDNARRLTGFHETSNINDFSAGVANRSASIRIPRTVGQEKKGYFEDRRPSANCDPFSVTEALIRTCLLNETGDEPFQYKN
Gene ID2752
Swiss protP15104
SynonymsGLNS; GS; PIG43; PIG59; GLUL
Calculated MW42kDa
Observed MW40kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesK-562, U-87MG, Mouse intestine, Mouse kidney
Cellular locationCytoplasm, Mitochondrion
Customer validation

(MCF-7)

ABclonal:Western blot - [KO Validated] GLUL Rabbit pAb (A5437) }

Western blot - [KO Validated] GLUL Rabbit pAb (A5437)

Western blot analysis of extracts of various cell lines, using GLUL antibody (A5437) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Western blot - [KO Validated] GLUL Rabbit pAb (A5437) }

Western blot - [KO Validated] GLUL Rabbit pAb (A5437)

Western blot analysis of extracts from normal (control) and GLUL knockout (KO) HeLa cells, using GLUL antibody (A5437) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.
ABclonal:Immunohistochemistry - [KO Validated] GLUL Rabbit pAb (A5437) }

Immunohistochemistry - [KO Validated] GLUL Rabbit pAb (A5437)

Immunohistochemistry of paraffin-embedded human esophageal using [KO Validated] GLUL Rabbit pAb (A5437) at dilution of 1:150 (40x lens).
ABclonal:Immunohistochemistry - [KO Validated] GLUL Rabbit pAb (A5437) }

Immunohistochemistry - [KO Validated] GLUL Rabbit pAb (A5437)

Immunohistochemistry of paraffin-embedded human liver using [KO Validated] GLUL Rabbit pAb (A5437) at dilution of 1:150 (40x lens).
ABclonal:Immunohistochemistry - [KO Validated] GLUL Rabbit pAb (A5437) }

Immunohistochemistry - [KO Validated] GLUL Rabbit pAb (A5437)

Immunohistochemistry of paraffin-embedded rat liver using [KO Validated] GLUL Rabbit pAb (A5437) at dilution of 1:150 (40x lens).
ABclonal:Immunofluorescence - [KO Validated] GLUL Rabbit pAb (A5437) }

Immunofluorescence - [KO Validated] GLUL Rabbit pAb (A5437)

Immunofluorescence analysis of MCF-7 cells using GLUL antibody (A5437). Blue: DAPI for nuclear staining.

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