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[KO Validated] GIT1 Rabbit pAb (A15437)

Datasheet

ABclonal:Western blot - [KO Validated] GIT1 Polyclonal Antibody (A15437)
ABclonal:Western blot - [KO Validated] GIT1 Polyclonal Antibody (A15437)
ABclonal:Immunofluorescence - [KO Validated] GIT1 Polyclonal Antibody (A15437)
ABclonal:Immunofluorescence - [KO Validated] GIT1 Polyclonal Antibody (A15437)
ABclonal:Immunofluorescence - [KO Validated] GIT1 Polyclonal Antibody (A15437)

Overview

Product name[KO Validated] GIT1 Rabbit pAb
Catalog No.A15437
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 460-640 of human GIT1 (NP_054749.2).
SequenceEIHKLQAENLQLRQPPGPVPTPPLPSERAEHTPMAPGGSTHRRDRQAFSMYEPGSALKPFGGPPGDELTTRLQPFHSTELEDDAIYSVHVPAGLYRIRKGVSASAVPFTPSSPLLSCSQEGSRHTSKLSRHGSGADSDYENTQSGDPLLGLEGKRFLELGKEEDFHPELESLDGDLDPGLP
Gene ID28964
Swiss protQ9Y2X7
SynonymsGIT1
Calculated MW19kDa/84kDa/85kDa
Observed MW100kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:200 - 1:2000
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunofluorescence    
Positive samplesU-251MG, A-549, A375, Mouse heart, Rat testis
Cellular locationCytoplasm
ABclonal:Western blot - [KO Validated] GIT1 Polyclonal Antibody (A15437) }

Western blot - [KO Validated] GIT1 Polyclonal Antibody (A15437)

Western blot analysis of extracts of various cell lines, using GIT1 antibody (A15437) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Western blot - [KO Validated] GIT1 Polyclonal Antibody (A15437) }

Western blot - [KO Validated] GIT1 Polyclonal Antibody (A15437)

Western blot analysis of extracts from normal (control) and GIT1 knockout (KO) HeLa cells, using GIT1 antibody (A15437) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Immunofluorescence - [KO Validated] GIT1 Polyclonal Antibody (A15437) }

Immunofluorescence - [KO Validated] GIT1 Polyclonal Antibody (A15437)

Immunofluorescence analysis of C6 cells using [KO] GIT1 antibody (A15437) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - [KO Validated] GIT1 Polyclonal Antibody (A15437) }

Immunofluorescence - [KO Validated] GIT1 Polyclonal Antibody (A15437)

Immunofluorescence analysis of L929 cells using [KO] GIT1 antibody (A15437) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - [KO Validated] GIT1 Polyclonal Antibody (A15437) }

Immunofluorescence - [KO Validated] GIT1 Polyclonal Antibody (A15437)

Immunofluorescence analysis of U-2 OS cells using [KO] GIT1 antibody (A15437) at dilution of 1:100. Blue: DAPI for nuclear staining.

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