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[KO Validated] GDI2 Rabbit pAb (A8615)

Datasheet

ABclonal:Western blot - [KO Validated] GDI2 Polyclonal Antibody (A8615)
ABclonal:Western blot - [KO Validated] GDI2 Polyclonal Antibody (A8615)
ABclonal:Immunofluorescence - [KO Validated] GDI2 Polyclonal Antibody (A8615)

Overview

Product name[KO Validated] GDI2 Rabbit pAb
Catalog No.A8615
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
GDP dissociation inhibitors are proteins that regulate the GDP-GTP exchange reaction of members of the rab family, small GTP-binding proteins of the ras superfamily, that are involved in vesicular trafficking of molecules between cellular organelles. GDIs slow the rate of dissociation of GDP from rab proteins and release GDP from membrane-bound rabs. GDI2 is ubiquitously expressed. The GDI2 gene contains many repetitive elements indicating that it may be prone to inversion/deletion rearrangements. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 296-445 of human GDI2 (NP_001485.2).
SequenceQVIRVICILSHPIKNTNDANSCQIIIPQNQVNRKSDIYVCMISFAHNVAAQGKYIAIVSTTVETKEPEKEIRPALELLEPIEQKFVSISDLLVPKDLGTESQIFISRTYDATTHFETTCDDIKNIYKRMTGSEFDFEEMKRKKNDIYGED
Gene ID2665
Swiss protP50395
SynonymsGDI2; HEL-S-46e; RABGDIB
Calculated MW45kDa/50kDa
Observed MW51kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:200 - 1:3000
IF 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunofluorescence    
Positive samplesHT-29, A-549, HepG2, U-87MG, Mouse spleen, Mouse kidney, Mouse lung, Rat liver, Rat spinal cord
Cellular locationCytoplasm, Membrane, Peripheral membrane protein
    ABclonal:Western blot - [KO Validated] GDI2 Polyclonal Antibody (A8615) }

    Western blot - [KO Validated] GDI2 Polyclonal Antibody (A8615)

    Western blot analysis of extracts of various cell lines, using GDI2 antibody (A8615) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25ug per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 1s.
    ABclonal:Western blot - [KO Validated] GDI2 Polyclonal Antibody (A8615) }

    Western blot - [KO Validated] GDI2 Polyclonal Antibody (A8615)

    Western blot analysis of extracts from normal (control) and GDI2 knockout (KO) 293T cells, using GDI2 antibody (A8615) at 1:2000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25ug per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 1s.
    ABclonal:Immunofluorescence - [KO Validated] GDI2 Polyclonal Antibody (A8615) }

    Immunofluorescence - [KO Validated] GDI2 Polyclonal Antibody (A8615)

    Immunofluorescence analysis of NIH/3T3 cells using GDI2 antibody (A8615) at dilution of 1:100. Blue: DAPI for nuclear staining.

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