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[KO Validated] Fatty Acid Synthase Rabbit pAb (A0462)

Publication (1) Datasheet

ABclonal:Western blot - [KO Validated] Fatty Acid Synthase Rabbit pAb (A0462)
ABclonal:Western blot - [KO Validated] Fatty Acid Synthase Rabbit pAb (A0462)
ABclonal:Immunohistochemistry - [KO Validated] Fatty Acid Synthase Rabbit pAb (A0462)
ABclonal:Immunohistochemistry - [KO Validated] Fatty Acid Synthase Rabbit pAb (A0462)
ABclonal:Immunofluorescence - [KO Validated] Fatty Acid Synthase Rabbit pAb (A0462)
ABclonal:Immunoprecipitation - [KO Validated] Fatty Acid Synthase Rabbit pAb (A0462)

Overview

Product name[KO Validated] Fatty Acid Synthase Rabbit pAb
Catalog No.A0462
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The enzyme encoded by this gene is a multifunctional protein. Its main function is to catalyze the synthesis of palmitate from acetyl-CoA and malonyl-CoA, in the presence of NADPH, into long-chain saturated fatty acids. In some cancer cell lines, this protein has been found to be fused with estrogen receptor-alpha (ER-alpha), in which the N-terminus of FAS is fused in-frame with the C-terminus of ER-alpha.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 2212-2511 of human Fatty Acid Synthase (NP_004095.4).
SequenceQQQTQLNLRSLLVNPEGPTLMRLNSVQSSERPLFLVHPIEGSTTVFHSLASRLSIPTYGLQCTRAAPLDSIHSLAAYYIDCIRQVQPEGPYRVAGYSYGACVAFEMCSQLQAQQSPAPTHNSLFLFDGSPTYVLAYTQSYRAKLTPGCEAEAETEAICFFVQQFTDMEHNRVLEALLPLKGLEERVAAAVDLIIKSHQGLDRQELSFAARSFYYKLRAAEQYTPKAKYHGNVMLLRAKTGGAYGEDLGADYNLSQVCDGKVSVHVIEGDHRTLLEGSGLESIISIIHSSLAEPRVSVREG
Gene ID2194
Swiss protP49327
SynonymsFAS; OA-519; SDR27X1; FASN
Calculated MW273kDa
Observed MW275kDa
ReactivityHuman
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:100
IF 1:50 - 1:200
IP 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive samplesH460
Cellular locationCytoplasm, Melanosome
Customer validation

WB(Homo sapiens)

ABclonal:Western blot - [KO Validated] Fatty Acid Synthase Rabbit pAb (A0462) }

Western blot - [KO Validated] Fatty Acid Synthase Rabbit pAb (A0462)

Western blot analysis of extracts of H460 cells, using Fatty Acid Synthase antibody (A0462) at 1:200 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Western blot - [KO Validated] Fatty Acid Synthase Rabbit pAb (A0462) }

Western blot - [KO Validated] Fatty Acid Synthase Rabbit pAb (A0462)

Western blot analysis of extracts from normal (control) and Fatty Acid Synthase knockout (KO) 293T cells, using Fatty Acid Synthase antibody (A0462) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunohistochemistry - [KO Validated] Fatty Acid Synthase Rabbit pAb (A0462) }

Immunohistochemistry - [KO Validated] Fatty Acid Synthase Rabbit pAb (A0462)

Immunohistochemistry of paraffin-embedded human colon using Fatty Acid Synthase antibody (A0462) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - [KO Validated] Fatty Acid Synthase Rabbit pAb (A0462) }

Immunohistochemistry - [KO Validated] Fatty Acid Synthase Rabbit pAb (A0462)

Immunohistochemistry of paraffin-embedded human gastric cancer using Fatty Acid Synthase antibody (A0462) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - [KO Validated] Fatty Acid Synthase Rabbit pAb (A0462) }

Immunofluorescence - [KO Validated] Fatty Acid Synthase Rabbit pAb (A0462)

Immunofluorescence analysis of A549 cells using Fatty Acid Synthase antibody (A0462). Blue: DAPI for nuclear staining.
ABclonal:Immunoprecipitation - [KO Validated] Fatty Acid Synthase Rabbit pAb (A0462) }

Immunoprecipitation - [KO Validated] Fatty Acid Synthase Rabbit pAb (A0462)

Immunoprecipitation analysis of 200ug extracts of H460 cells, using 3 ug Fatty Acid Synthase antibody (A0462). Western blot was performed from the immunoprecipitate using Fatty Acid Synthase antibody (A0462) at a dilition of 1:1000.

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