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[KO Validated] FUS Rabbit pAb (A5921)

Datasheet

ABclonal:Western blot - [KO Validated] FUS Rabbit pAb (A5921)
ABclonal:Western blot - [KO Validated] FUS Rabbit pAb (A5921)
ABclonal:Immunohistochemistry - [KO Validated] FUS Rabbit pAb (A5921)
ABclonal:Immunohistochemistry - [KO Validated] FUS Rabbit pAb (A5921)
ABclonal:Immunohistochemistry - [KO Validated] FUS Rabbit pAb (A5921)
ABclonal:Immunohistochemistry - [KO Validated] FUS Rabbit pAb (A5921)
ABclonal:Immunofluorescence - [KO Validated] FUS Rabbit pAb (A5921)
ABclonal:Immunofluorescence - [KO Validated] FUS Rabbit pAb (A5921)
ABclonal:Immunoprecipitation - [KO Validated] FUS Rabbit pAb (A5921)

Overview

Product name[KO Validated] FUS Rabbit pAb
Catalog No.A5921
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a multifunctional protein component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complex. The hnRNP complex is involved in pre-mRNA splicing and the export of fully processed mRNA to the cytoplasm. This protein belongs to the FET family of RNA-binding proteins which have been implicated in cellular processes that include regulation of gene expression, maintenance of genomic integrity and mRNA/microRNA processing. Alternative splicing results in multiple transcript variants. Defects in this gene result in amyotrophic lateral sclerosis type 6.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 297-526 of human FUS (NP_004951.1).
SequenceTIESVADYFKQIGIIKTNKKTGQPMINLYTDRETGKLKGEATVSFDDPPSAKAAIDWFDGKEFSGNPIKVSFATRRADFNRGGGNGRGGRGRGGPMGRGGYGGGGSGGGGRGGFPSGGGGGGGQQRAGDWKCPNPTCENMNFSWRNECNQCKAPKPDGPGGGPGGSHMGGNYGDDRRGGRGGYDRGGYRGRGGDRGGFRGGRGGGDRGGFGPGKMDSRGEHRQDRRERPY
Gene ID2521
Swiss protP35637
SynonymsFUS; ALS6; ETM4; FUS1; HNRNPP2; POMP75; TLS
Calculated MW53kDa
Observed MW70kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:100
IF 1:50 - 1:100
IP 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive samplesU-937, Jurkat
Cellular locationNucleus
ABclonal:Western blot - [KO Validated] FUS Rabbit pAb (A5921) }

Western blot - [KO Validated] FUS Rabbit pAb (A5921)

Western blot analysis of extracts of various cell lines, using FUS antibody (A5921) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Western blot - [KO Validated] FUS Rabbit pAb (A5921) }

Western blot - [KO Validated] FUS Rabbit pAb (A5921)

Western blot analysis of extracts from normal (control) and FUS knockout (KO) 293T cells, using FUS antibody (A5921) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Immunohistochemistry - [KO Validated] FUS Rabbit pAb (A5921) }

Immunohistochemistry - [KO Validated] FUS Rabbit pAb (A5921)

Immunohistochemistry of paraffin-embedded rat brain using FUS antibody (A5921) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - [KO Validated] FUS Rabbit pAb (A5921) }

Immunohistochemistry - [KO Validated] FUS Rabbit pAb (A5921)

Immunohistochemistry of paraffin-embedded human breast cancer using FUS antibody (A5921) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - [KO Validated] FUS Rabbit pAb (A5921) }

Immunohistochemistry - [KO Validated] FUS Rabbit pAb (A5921)

Immunohistochemistry of paraffin-embedded mouse brain using FUS antibody (A5921) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - [KO Validated] FUS Rabbit pAb (A5921) }

Immunohistochemistry - [KO Validated] FUS Rabbit pAb (A5921)

Immunohistochemistry of paraffin-embedded mouse spleen using FUS antibody (A5921) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - [KO Validated] FUS Rabbit pAb (A5921) }

Immunofluorescence - [KO Validated] FUS Rabbit pAb (A5921)

Immunofluorescence analysis of C6 cells using FUS antibody (A5921) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - [KO Validated] FUS Rabbit pAb (A5921) }

Immunofluorescence - [KO Validated] FUS Rabbit pAb (A5921)

Immunofluorescence analysis of NIH/3T3 cells using FUS antibody (A5921) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Immunoprecipitation - [KO Validated] FUS Rabbit pAb (A5921) }

Immunoprecipitation - [KO Validated] FUS Rabbit pAb (A5921)

Immunoprecipitation analysis of 300ug extracts of Jurkat cells using 3ug FUS antibody (A5921). Western blot was performed from the immunoprecipitate using FUS antibody (A5921) at a dilition of 1:2000.

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