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[KO Validated] FSCN1 Rabbit pAb (A1904)

Review (1) Datasheet

ABclonal:Western blot - [KO Validated] FSCN1 Rabbit pAb (A1904)
ABclonal:Western blot - [KO Validated] FSCN1 Rabbit pAb (A1904)
ABclonal:Immunohistochemistry - [KO Validated] FSCN1 Rabbit pAb (A1904)
ABclonal:Immunofluorescence - [KO Validated] FSCN1 Rabbit pAb (A1904)
ABclonal:Immunofluorescence - [KO Validated] FSCN1 Rabbit pAb (A1904)
Review
ABclonal: review for [KO Validated] FSCN1 Rabbit pAb(A1904)

Overview

Product name[KO Validated] FSCN1 Rabbit pAb
Catalog No.A1904
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the fascin family of actin-binding proteins. Fascin proteins organize F-actin into parallel bundles, and are required for the formation of actin-based cellular protrusions. The encoded protein plays a critical role in cell migration, motility, adhesion and cellular interactions. Expression of this gene is known to be regulated by several microRNAs, and overexpression of this gene may play a role in the metastasis of multiple types of cancer by increasing cell motility. Expression of this gene is also a marker for Reed-Sternberg cells in Hodgkin's lymphoma. A pseudogene of this gene is located on the long arm of chromosome 15.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 394-493 of human FSCN1 (NP_003079.1).
SequenceFIGCRKVTGTLDANRSSYDVFQLEFNDGAYNIKDSTGKYWTVGSDSAVTSSGDTPVDFFFEFCDYNKVAIKVGGRYLKGDHAGVLKASAETVDPASLWEY
Gene ID6624
Swiss protQ16658
SynonymsFSCN1; FAN1; HSN; SNL; p55; fascin
Calculated MW54kDa
Observed MW50kDa, 55kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesU-251MG, HeLa, NCI-H460, HepG2, SH-SY5Y, Mouse brain, Mouse spinal cord
Cellular locationCell junction, Cell projection, Cytoplasm, cytoskeleton, cytosol, filopodium, invadopodium, microvillus
Customer validation

(Mouse keRatinocytes)

ABclonal:Western blot - [KO Validated] FSCN1 Rabbit pAb (A1904) }

Western blot - [KO Validated] FSCN1 Rabbit pAb (A1904)

Western blot analysis of extracts of various cell lines, using FSCN1 antibody (A1904) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Western blot - [KO Validated] FSCN1 Rabbit pAb (A1904) }

Western blot - [KO Validated] FSCN1 Rabbit pAb (A1904)

Western blot analysis of extracts from normal (control) and FSCN1 knockout (KO) HeLa cells, using FSCN1 antibody (A1904) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.
ABclonal:Immunohistochemistry - [KO Validated] FSCN1 Rabbit pAb (A1904) }

Immunohistochemistry - [KO Validated] FSCN1 Rabbit pAb (A1904)

Immunohistochemistry of paraffin-embedded mouse testis using [KO Validated] FSCN1 Rabbit pAb (A1904) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - [KO Validated] FSCN1 Rabbit pAb (A1904) }

Immunofluorescence - [KO Validated] FSCN1 Rabbit pAb (A1904)

Immunofluorescence analysis of C6 cells using [KO Validated] FSCN1 Rabbit pAb (A1904) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - [KO Validated] FSCN1 Rabbit pAb (A1904) }

Immunofluorescence - [KO Validated] FSCN1 Rabbit pAb (A1904)

Immunofluorescence analysis of NIH-3T3 cells using [KO Validated] FSCN1 Rabbit pAb (A1904) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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