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[KO Validated] Eif2ak2 Rabbit pAb (A5578)

Datasheet

ABclonal:Western blot - [KO Validated] Eif2ak2 Polyclonal Antibody (A5578)
ABclonal:Immunohistochemistry - [KO Validated] Eif2ak2 Polyclonal Antibody (A5578)
ABclonal:Immunofluorescence - [KO Validated] Eif2ak2 Polyclonal Antibody (A5578)

Overview

Product name[KO Validated] Eif2ak2 Rabbit pAb
Catalog No.A5578
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a serine/threonine protein kinase that is activated by autophosphorylation after binding to dsRNA. The activated form of the encoded protein can phosphorylate translation initiation factor EIF2S1, which in turn inhibits protein synthesis. This protein is also activated by manganese ions and heparin. Three transcript variants encoding two different isoforms have been found for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human Eif2ak2 (NP_002750.1).
SequenceMAGDLSAGFFMEELNTYRQKQGVVLKYQELPNSGPPHDRRFTFQVIIDGREFPEGEGRSKKEAKNAAAKLAVEILNKEKKAVSPLLLTTTNSSEGLSMGNYIGLINRIAQKKRLTVNYEQCASGVHGPEGFHYKCKMGQKEYSIGTGSTKQEAKQLAAKLAYLQILSEETSVKSDYLSSGSFATTCESQSNSLVTSTLASESSSEGDFSADTSEINSNSDSLNSSSLLMNGLRNNQRKAKRSLAPRFDLPDMKETKYTVDKRFGMDFKEI
Gene ID5610
Swiss protP19525
SynonymsEIF2AK1; PKR; PPP1R83; PRKR; Eif2ak2; EIF2AK2
Calculated MW57kDa/62kDa
Observed MW74kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
IHC 1:100 - 1:200
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesHeLa, Jurkat
Cellular locationCytoplasm, Nucleus, perinuclear region
ABclonal:Western blot - [KO Validated] Eif2ak2 Polyclonal Antibody (A5578) }

Western blot - [KO Validated] Eif2ak2 Polyclonal Antibody (A5578)

Western blot analysis of extracts of various cell lines, using Eif2ak2 antibody (A5578) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Immunohistochemistry - [KO Validated] Eif2ak2 Polyclonal Antibody (A5578) }

Immunohistochemistry - [KO Validated] Eif2ak2 Polyclonal Antibody (A5578)

Immunohistochemistry of paraffin-embedded human liver using Eif2ak2 antibody (A5578) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - [KO Validated] Eif2ak2 Polyclonal Antibody (A5578) }

Immunofluorescence - [KO Validated] Eif2ak2 Polyclonal Antibody (A5578)

Immunofluorescence analysis of U2OS cells using EIF2AK2 antibody (A5578) at dilution of 1:100. Blue: DAPI for nuclear staining.

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