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[KO Validated] ERP29 Rabbit pAb (A7959)

Datasheet

ABclonal:Western blot - [KO Validated] ERP29 Polyclonal Antibody (A7959)
ABclonal:Western blot - [KO Validated] ERP29 Polyclonal Antibody (A7959)

Overview

Product name[KO Validated] ERP29 Rabbit pAb
Catalog No.A7959
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a protein which localizes to the lumen of the endoplasmic reticulum (ER). It is a member of the protein disulfide isomerase (PDI) protein family but lacks an active thioredoxin motif, suggesting that this protein does not function as a disulfide isomerase. The canonical protein dimerizes and is thought to play a role in the processing of secretory proteins within the ER. Alternative splicing results in multiple transcript variants encoding different isoforms.
ImmunogenA synthetic peptide of human ERP29
SequenceEmail for sequence
Gene ID10961
Swiss protP30040
SynonymsERP29; C12orf8; ERp28; ERp31; HEL-S-107; PDI-DB; PDIA9
Calculated MW5kDa/28kDa
Observed MW29kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    
Positive samplesHL-60, THP-1, HeLa, 293T, Mouse testis, Mouse pancreas, Mouse thymus, Mouse spleen
Cellular locationEndoplasmic reticulum lumen, Melanosome
    ABclonal:Western blot - [KO Validated] ERP29 Polyclonal Antibody (A7959) }

    Western blot - [KO Validated] ERP29 Polyclonal Antibody (A7959)

    Western blot analysis of extracts of various cell lines, using ERP29 antibody (A7959) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25ug per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Enhanced Kit (RM00021).
    Exposure time: 90s.
    ABclonal:Western blot - [KO Validated] ERP29 Polyclonal Antibody (A7959) }

    Western blot - [KO Validated] ERP29 Polyclonal Antibody (A7959)

    Western blot analysis of extracts from normal (control) and ERP29 knockout (KO) 293T cells, using ERP29 antibody (A7959) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25ug per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 5s.

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