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[KO Validated] ERK2 Polyclonal Antibody (A0229)

Reviews (4)Publications (8) Datasheet

ABclonal:Western blot - [KO Validated] ERK2 Polyclonal Antibody (A0229)
ABclonal:Western blot - [KO Validated] ERK2 Polyclonal Antibody (A0229)
Review
ABclonal: review for [KO Validated] ERK2 Polyclonal Antibody(A0229)
Review
ABclonal: review for [KO Validated] ERK2 Polyclonal Antibody(A0229)
Review
ABclonal: review for [KO Validated] ERK2 Polyclonal Antibody(A0229)
Review
ABclonal: review for [KO Validated] ERK2 Polyclonal Antibody(A0229)

Overview

Product name[KO Validated] ERK2 Polyclonal Antibody
Catalog No.A0229
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets. One study also suggests that this protein acts as a transcriptional repressor independent of its kinase activity. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Two alternatively spliced transcript variants encoding the same protein, but differing in the UTRs, have been reported for this gene.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 200-300 of human ERK2 (NP_620407.1).
SequenceLNSKGYTKSIDIWSVGCILAEMLSNRPIFPGKHYLDQLNHILGILGSPSQEDLNCIINLKARNYLLSLPHKNKVPWNRLFPNADSKALDLLDKMLTFNPHK
Gene ID5594
Swiss protP28482
SynonymsMAPK1; ERK; ERK-2; ERK2; ERT1; MAPK2; P42MAPK; PRKM1; PRKM2; p38; p40; p41; p41mapk; p42-MAPK; mitogen-activated protein kinase 1
Calculated MW36kDa/41kDa
Observed MW41kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide Array
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    
Positive samplesHeLa, Jurkat, Mouse brain, Mouse spleen, Rat brain, Rat spinal cord
Cellular locationCytoplasm, Nucleus, centrosome, cytoskeleton, microtubule organizing center, spindle
Customer validation

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WB(Rat, Human, Mouse, Rat)

ABclonal:Western blot - [KO Validated] ERK2 Polyclonal Antibody (A0229) }

Western blot - [KO Validated] ERK2 Polyclonal Antibody (A0229)

Western blot analysis of extracts of various cell lines, using ERK2 antibody (A0229) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
ABclonal:Western blot - [KO Validated] ERK2 Polyclonal Antibody (A0229) }

Western blot - [KO Validated] ERK2 Polyclonal Antibody (A0229)

Western blot analysis of extracts from normal (control) and ERK2 knockout (KO) HeLa cells, using ERK2 antibody (A0229) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5min.

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