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[KO Validated] EIF4EBP1 Polyclonal Antibody (A1248)

Publications (3) Datasheet

ABclonal:Western blot - [KO Validated] EIF4EBP1 Polyclonal Antibody (A1248)
ABclonal:Western blot - [KO Validated] EIF4EBP1 Polyclonal Antibody (A1248)
ABclonal:Immunofluorescence - [KO Validated] EIF4EBP1 Polyclonal Antibody (A1248)

Overview

Product name[KO Validated] EIF4EBP1 Polyclonal Antibody
Catalog No.A1248
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 50 to the C-terminus of human EIF4EBP1 (NP_004086.1).
SequenceTRIIYDRKFLMECRNSPVTKTPPRDLPTIPGVTSPSSDEPPMEASQSHLRNSPEDKRAGGEESQFEMDI
Gene ID1978
Swiss protQ13541
SynonymsEIF4EBP1; 4E-BP1; 4EBP1; BP-1; PHAS-I; eukaryotic translation initiation factor 4E-binding protein 1
Calculated MW12kDa
Observed MW17kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFC
Recommended dilutionWB 1:500 - 1:1000
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunofluorescence    
Positive samplesMCF7, HeLa, SH-SY5Y, NIH/3T3, Mouse fat, Mouse heart
Cellular location
Customer validation

WB(African green monkey, Human, Mouse)

ABclonal:Western blot - [KO Validated] EIF4EBP1 Polyclonal Antibody (A1248) }

Western blot - [KO Validated] EIF4EBP1 Polyclonal Antibody (A1248)

Western blot analysis of extracts of various cell lines, using EIF4EBP1 antibody (A1248) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
ABclonal:Western blot - [KO Validated] EIF4EBP1 Polyclonal Antibody (A1248) }

Western blot - [KO Validated] EIF4EBP1 Polyclonal Antibody (A1248)

Western blot analysis of extracts from normal (control) and EIF4EBP1 knockout (KO) HeLa cells, using EIF4EBP1 antibody (A1248) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Immunofluorescence - [KO Validated] EIF4EBP1 Polyclonal Antibody (A1248) }

Immunofluorescence - [KO Validated] EIF4EBP1 Polyclonal Antibody (A1248)

Immunofluorescence analysis of NIH/3T3 cells using EIF4EBP1 antibody (A1248) at dilution of 1:100. Blue: DAPI for nuclear staining.

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