Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

EGFR Rabbit pAb (A11351)

Reviews (2) Publications (30) Datasheet COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - EGFR Rabbit pAb (A11351)

Western blot analysis of lysates from A431 cells, using EGFR Rabbit pAb (A11351) at 1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.

ABclonal:Immunohistochemistry - EGFR Rabbit pAb (A11351)

Immunohistochemistry analysis of EGFR in paraffin-embedded Rat liver using EGFR Rabbit pAb (A11351) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - EGFR Rabbit pAb (A11351)

Immunohistochemistry analysis of EGFR in paraffin-embedded Human placenta using EGFR Rabbit pAb (A11351) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - EGFR Rabbit pAb (A11351)

Immunohistochemistry analysis of EGFR in paraffin-embedded Mouse heart using EGFR Rabbit pAb (A11351) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - EGFR Rabbit pAb (A11351)

Immunofluorescence analysis of A-431 cells using EGFR Rabbit pAb (A11351) at dilution of 1:300 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunoprecipitation - EGFR Rabbit pAb (A11351)

Immunoprecipitation analysis of 200 μg extracts of HeLa cells using 3 μg EGFR antibody (A11351). Western blot was performed from the immunoprecipitate using EGFR antibody (A11351) at a dilution of 1:1000.

Review
ABclonal: review for EGFR Rabbit pAb(A11351)
Review
ABclonal: review for EGFR Rabbit pAb(A11351)

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Overview

Product name EGFR Rabbit pAb
Catalog No. A11351
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor, thus inducing receptor dimerization and tyrosine autophosphorylation leading to cell proliferation. Mutations in this gene are associated with lung cancer. EGFR is a component of the cytokine storm which contributes to a severe form of Coronavirus Disease 2019 (COVID-19) resulting from infection with severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2).

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1021-1210 of human EGFR (NP_005219.2).
Sequence QGFFSSPSTSRTPLLSSLSATSNNSTVACIDRNGLQSCPIKEDSFLQRYSSDPTGALTEDSIDDTFLPVPEYINQSVPKRPAGSVQNPVYHNQPLNPAPSRDPHYQDPHSTAVGNPEYLNTVQPTCVNSTFDSPAHWAQKGSHQISLDNPDYQQDFFPKEAKPNGIFKGSTAENAEYLRVAPQSSEFIGA
Gene ID 1956
Swiss prot P00533
Synonyms ERBB; ERRP; HER1; mENA; ERBB1; PIG61; NISBD2; EGFR
Calculated MW 134kDa
Observed MW 175kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:1000 - 1:5000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:100 - 1:500
  • IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive samples A431
Cellular location Cell membrane, Endoplasmic reticulum membrane, Endosome, Endosome membrane, Golgi apparatus membrane, Nucleus membrane, Nucleus, Secreted, Single-pass type I membrane protein
Customer validation

WB (H1975Mus musculus, Zika virus, Homo sapiens, Mus musculus)

IHC (Human gliomaHomo sapiens, Homo sapiens, Mus musculus)

IF (Homo sapiens, Mus musculus)

IP (Homo sapiens)

FC (Homo sapiens)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

EGFR Rabbit pAb images

ABclonal:Western blot - EGFR Rabbit pAb (A11351)}

Western blot - EGFR Rabbit pAb (A11351)

Western blot analysis of lysates from A431 cells, using EGFR Rabbit pAb (A11351) at 1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.
ABclonal:Immunohistochemistry - EGFR Rabbit pAb (A11351)}

Immunohistochemistry - EGFR Rabbit pAb (A11351)

Immunohistochemistry analysis of EGFR in paraffin-embedded Rat liver using EGFR Rabbit pAb (A11351) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - EGFR Rabbit pAb (A11351)}

Immunohistochemistry - EGFR Rabbit pAb (A11351)

Immunohistochemistry analysis of EGFR in paraffin-embedded Human placenta using EGFR Rabbit pAb (A11351) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - EGFR Rabbit pAb (A11351)}

Immunohistochemistry - EGFR Rabbit pAb (A11351)

Immunohistochemistry analysis of EGFR in paraffin-embedded Mouse heart using EGFR Rabbit pAb (A11351) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - EGFR Rabbit pAb (A11351)}

Immunofluorescence - EGFR Rabbit pAb (A11351)

Immunofluorescence analysis of A-431 cells using EGFR Rabbit pAb (A11351) at dilution of 1:300 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunoprecipitation - EGFR Rabbit pAb (A11351)}

Immunoprecipitation - EGFR Rabbit pAb (A11351)

Immunoprecipitation analysis of 200 μg extracts of HeLa cells using 3 μg EGFR antibody (A11351). Western blot was performed from the immunoprecipitate using EGFR antibody (A11351) at a dilution of 1:1000.

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Reviews List

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Related Targets

Based on existing publications, the following genes are closely related to research on EGFR. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to EGFR. (Distance between topics and target gene indicate popularity.) EGFR

* Data provided by citexs.com, for reference only.

Publishing research using A11351? Please let us know so that we can cite the reference in this datasheet.

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