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[KO Validated] EGFR Polyclonal Antibody (A11351)

Reviews (2) Datasheet

ABclonal:Western blot - [KO Validated] EGFR Polyclonal Antibody (A11351)
ABclonal:Western blot - [KO Validated] EGFR Polyclonal Antibody (A11351)
ABclonal:Immunohistochemistry - [KO Validated] EGFR Polyclonal Antibody (A11351)
ABclonal:Immunohistochemistry - [KO Validated] EGFR Polyclonal Antibody (A11351)
ABclonal:Immunofluorescence - [KO Validated] EGFR Polyclonal Antibody (A11351)
ABclonal:Immunofluorescence - [KO Validated] EGFR Polyclonal Antibody (A11351)
ABclonal:Immunofluorescence - [KO Validated] EGFR Polyclonal Antibody (A11351)
ABclonal:Immunoprecipitation - [KO Validated] EGFR Polyclonal Antibody (A11351)
Review
ABclonal:Western blot (WB) review for [KO Validated] EGFR Polyclonal Antibody(A11351)
Review
ABclonal:Immunohistochemistry (IHC) review for [KO Validated] EGFR Polyclonal Antibody(A11351)

Overview

Product name[KO Validated] EGFR Polyclonal Antibody
Catalog No.A11351
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1021-1210 of human EGFR (NP_005219.2).
SequenceQGFFSSPSTSRTPLLSSLSATSNNSTVACIDRNGLQSCPIKEDSFLQRYSSDPTGALTEDSIDDTFLPVPEYINQSVPKRPAGSVQNPVYHNQPLNPAPSRDPHYQDPHSTAVGNPEYLNTVQPTCVNSTFDSPAHWAQKGSHQISLDNPDYQQDFFPKEAKPNGIFKGSTAENAEYLRVAPQSSEFIGA
Gene ID1956
Swiss protP00533
SynonymsEGFR; ERBB; ERBB1; HER1; NISBD2; PIG61; mENA; epidermal growth factor receptor
Calculated MW44kDa/69kDa/77kDa/134kDa
Observed MW160kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFC
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IP 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive samplesOVCAR-3, HeLa, HepG2, Mouse liver
Cellular locationCell membrane, Endoplasmic reticulum membrane, Endosome, Endosome membrane, Golgi apparatus membrane, Nucleus membrane, Nucleus, Secreted, Single-pass type I membrane protein
Customer validation

WB(H1975)

IHC(Human glioma)

ABclonal:Western blot - [KO Validated] EGFR Polyclonal Antibody (A11351) }

Western blot - [KO Validated] EGFR Polyclonal Antibody (A11351)

Western blot analysis of extracts of various cell lines, using EGFR antibody (A11351) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.
ABclonal:Western blot - [KO Validated] EGFR Polyclonal Antibody (A11351) }

Western blot - [KO Validated] EGFR Polyclonal Antibody (A11351)

Western blot analysis of extracts from normal (control) and EGFR knockout (KO) HeLa cells, using EGFR antibody (A11351) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Immunohistochemistry - [KO Validated] EGFR Polyclonal Antibody (A11351) }

Immunohistochemistry - [KO Validated] EGFR Polyclonal Antibody (A11351)

Immunohistochemistry of paraffin-embedded human prostate using EGFR antibody (A11351) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - [KO Validated] EGFR Polyclonal Antibody (A11351) }

Immunohistochemistry - [KO Validated] EGFR Polyclonal Antibody (A11351)

Immunohistochemistry of paraffin-embedded mouse brain using EGFR antibody (A11351) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - [KO Validated] EGFR Polyclonal Antibody (A11351) }

Immunofluorescence - [KO Validated] EGFR Polyclonal Antibody (A11351)

Immunofluorescence analysis of HeLa cells using EGFR antibody (A11351) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - [KO Validated] EGFR Polyclonal Antibody (A11351) }

Immunofluorescence - [KO Validated] EGFR Polyclonal Antibody (A11351)

Immunofluorescence analysis of NIH/3T3 cells using EGFR antibody (A11351) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - [KO Validated] EGFR Polyclonal Antibody (A11351) }

Immunofluorescence - [KO Validated] EGFR Polyclonal Antibody (A11351)

Immunofluorescence analysis of PC12 cells using EGFR antibody (A11351) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Immunoprecipitation - [KO Validated] EGFR Polyclonal Antibody (A11351) }

Immunoprecipitation - [KO Validated] EGFR Polyclonal Antibody (A11351)

Immunoprecipitation analysis of 200ug extracts of HeLa cells using 3ug EGFR antibody (A11351). Western blot was performed from the immunoprecipitate using EGFR antibody (A11351) at a dilition of 1:1000.

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