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[KO Validated] EEF2K Rabbit pAb (A5404)

Datasheet

ABclonal:Western blot - [KO Validated] EEF2K Polyclonal Antibody (A5404)
ABclonal:Western blot - [KO Validated] EEF2K Polyclonal Antibody (A5404)
ABclonal:Immunofluorescence - [KO Validated] EEF2K Polyclonal Antibody (A5404)

Overview

Product name[KO Validated] EEF2K Rabbit pAb
Catalog No.A5404
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a highly conserved protein kinase in the calmodulin-mediated signaling pathway that links activation of cell surface receptors to cell division. This kinase is involved in the regulation of protein synthesis. It phosphorylates eukaryotic elongation factor 2 (EEF2) and thus inhibits the EEF2 function. The activity of this kinase is increased in many cancers and may be a valid target for anti-cancer treatment.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 406-725 of human EEF2K (NP_037434.1).
SequenceKLDHLHWPVFSDLDNMASRDHDHLDNHRESENSGDSGYPSEKRGELDDPEPREHGHSYSNRKYESDEDSLGSSGRVCVEKWNLLNSSRLHLPRASAVALEVQRLNALDLEKKIGKSILGKVHLAMVRYHEGGRFCEKGEEWDQESAVFHLEHAANLGELEAIVGLGLMYSQLPHHILADVSLKETEENKTKGFDYLLKAAEAGDRQSMILVARAFDSGQNLSPDRCQDWLEALHWYNTALEMTDCDEGGEYDGMQDEPRYMMLAREAEMLFTGGYGLEKDPQRSGDLYTQAAEAAMEAMKGRLANQYYQKAEEAWAQMEE
Gene ID29904
Swiss protO00418
SynonymsEEF2K; HSU93850; eEF-2K
Calculated MW82kDa
Observed MW110kDa
ReactivityHuman, Mouse
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunofluorescence    
Positive samplesHepG2, HeLa, LOVO, 22Rv1, 293T, Mouse spleen
Cellular location
ABclonal:Western blot - [KO Validated] EEF2K Polyclonal Antibody (A5404) }

Western blot - [KO Validated] EEF2K Polyclonal Antibody (A5404)

Western blot analysis of extracts of various cell lines, using EEF2K antibody (A5404) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 40s.
ABclonal:Western blot - [KO Validated] EEF2K Polyclonal Antibody (A5404) }

Western blot - [KO Validated] EEF2K Polyclonal Antibody (A5404)

Western blot analysis of extracts from normal (control) and EEF2K knockout (KO) HeLa cells, using EEF2K antibody (A5404) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Immunofluorescence - [KO Validated] EEF2K Polyclonal Antibody (A5404) }

Immunofluorescence - [KO Validated] EEF2K Polyclonal Antibody (A5404)

Immunofluorescence analysis of A-549 cells using EEF2K antibody (A5404). Blue: DAPI for nuclear staining.

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