Products > Catalog Antibodies > Polyclonal Antibodies

[KO Validated] EEF1B2 Rabbit pAb (A6580)

Datasheet

ABclonal:Western blot - [KO Validated] EEF1B2 Polyclonal Antibody (A6580)
ABclonal:Western blot - [KO Validated] EEF1B2 Polyclonal Antibody (A6580)
ABclonal:Immunohistochemistry - [KO Validated] EEF1B2 Polyclonal Antibody (A6580)
ABclonal:Immunohistochemistry - [KO Validated] EEF1B2 Polyclonal Antibody (A6580)
ABclonal:Immunohistochemistry - [KO Validated] EEF1B2 Polyclonal Antibody (A6580)
ABclonal:Immunofluorescence - [KO Validated] EEF1B2 Polyclonal Antibody (A6580)

Overview

Product name[KO Validated] EEF1B2 Rabbit pAb
Catalog No.A6580
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a translation elongation factor. The protein is a guanine nucleotide exchange factor involved in the transfer of aminoacylated tRNAs to the ribosome. Alternative splicing results in three transcript variants which differ only in the 5' UTR.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-225 of human EEF1B2 (NP_001032752.1).
SequenceMGFGDLKSPAGLQVLNDYLADKSYIEGYVPSQADVAVFEAVSSPPPADLCHALRWYNHIKSYEKEKASLPGVKKALGKYGPADVEDTTGSGATDSKDDDDIDLFGSDDEEESEEAKRLREERLAQYESKKAKKPALVAKSSILLDVKPWDDETDMAKLEECVRSIQADGLVWGSSKLVPVGYGIKKLQIQCVVEDDKVGTDMLEEQITAFEDYVQSMDVAAFNKI
Gene ID1933
Swiss protP24534
SynonymsEEF1B2; EEF1B; EEF1B1; EF1B
Calculated MW24kDa
Observed MW33kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesMCF-7, HL-60, HeLa, 293T, Mouse pancreas
Cellular location
    ABclonal:Western blot - [KO Validated] EEF1B2 Polyclonal Antibody (A6580) }

    Western blot - [KO Validated] EEF1B2 Polyclonal Antibody (A6580)

    Western blot analysis of extracts of various cell lines, using EEF1B2 antibody (A6580) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25ug per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 90s.
    ABclonal:Western blot - [KO Validated] EEF1B2 Polyclonal Antibody (A6580) }

    Western blot - [KO Validated] EEF1B2 Polyclonal Antibody (A6580)

    Western blot analysis of extracts from normal (control) and EEF1B2 knockout (KO) 293T cells, using EEF1B2 antibody (A6580) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25ug per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 30s.
    ABclonal:Immunohistochemistry - [KO Validated] EEF1B2 Polyclonal Antibody (A6580) }

    Immunohistochemistry - [KO Validated] EEF1B2 Polyclonal Antibody (A6580)

    Immunohistochemistry of paraffin-embedded rat spinal cord using EEF1B2 Antibody (A6580) at dilution of 1:100 (40x lens).
    ABclonal:Immunohistochemistry - [KO Validated] EEF1B2 Polyclonal Antibody (A6580) }

    Immunohistochemistry - [KO Validated] EEF1B2 Polyclonal Antibody (A6580)

    Immunohistochemistry of paraffin-embedded rat brain using EEF1B2 Antibody (A6580) at dilution of 1:100 (40x lens).
    ABclonal:Immunohistochemistry - [KO Validated] EEF1B2 Polyclonal Antibody (A6580) }

    Immunohistochemistry - [KO Validated] EEF1B2 Polyclonal Antibody (A6580)

    Immunohistochemistry of paraffin-embedded mouse brain using EEF1B2 Antibody (A6580) at dilution of 1:100 (40x lens).
    ABclonal:Immunofluorescence - [KO Validated] EEF1B2 Polyclonal Antibody (A6580) }

    Immunofluorescence - [KO Validated] EEF1B2 Polyclonal Antibody (A6580)

    Immunofluorescence analysis of NIH/3T3 cells using EEF1B2 antibody (A6580) at dilution of 1:100. Blue: DAPI for nuclear staining.

    Inquire About This Product

    Submit your question about A6580 below and we will get back to you within one business day.

    Alternatively, call us at 888.754.5670.

    Select size and quantity
    Contact us to order