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[KO Validated] Dnmt3a Rabbit mAb (A19659)

Datasheet

ABclonal:Western blot - [KO Validated] Dnmt3a Rabbit mAb (A19659)
ABclonal:Western blot - [KO Validated] Dnmt3a Rabbit mAb (A19659)

Overview

Product name[KO Validated] Dnmt3a Rabbit mAb
Catalog No.A19659
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a DNA methyltransferase that is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes to the cytoplasm and nucleus and its expression is developmentally regulated. [provided by RefSeq, Mar 2016]
ImmunogenA synthesized peptide derived from human Dnmt3a.
SequenceEmail for sequence
Gene ID1788
Swiss protQ9Y6K1
SynonymsDNMT3A; DNMT3A2; M.HsaIIIA; TBRS; DNMT3A
Calculated MW130kDa
Observed MW80KDa/140KDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Application keyWestern blotting    
Positive samplesK-562, Mouse thymus, Mouse testis, Rat testis, 293T
Cellular location
ABclonal:Western blot - [KO Validated] Dnmt3a Rabbit mAb (A19659) }

Western blot - [KO Validated] Dnmt3a Rabbit mAb (A19659)

Western blot analysis of extracts from normal (control) and Dnmt3a knockout (KO) 293T cells, using Dnmt3a antibody (A19659) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1min.
ABclonal:Western blot - [KO Validated] Dnmt3a Rabbit mAb (A19659) }

Western blot - [KO Validated] Dnmt3a Rabbit mAb (A19659)

Western blot analysis of extracts of various cell lines, using Dnmt3a antibody (A19659) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1min.

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