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[KO Validated] DNMT1 Rabbit pAb (A5495)

Reviews (10)Publications (7) Datasheet

ABclonal:Western blot - [KO Validated] DNMT1 Polyclonal Antibody (A5495)

Western blot analysis of extracts of various cell lines, using DNMT1 antibody (A5495) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Western blot - [KO Validated] DNMT1 Polyclonal Antibody (A5495)

Western blot analysis of extracts from normal (control) and DNMT1 knockout (KO) 293T cells, using DNMT1 antibody (A5495) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunofluorescence - [KO Validated] DNMT1 Polyclonal Antibody (A5495)

Immunofluorescence analysis of 293T cells using DNMT1 antibody (A5495) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

ABclonal:Immunoprecipitation - [KO Validated] DNMT1 Polyclonal Antibody (A5495)

Immunoprecipitation analysis of 200ug extracts of 293T cells using 3ug DNMT1 antibody (A5495). Western blot was performed from the immunoprecipitate using DNMT1 antibody (A5495) at a dilition of 1:1000.

Review
ABclonal: review for [KO Validated] DNMT1 Rabbit pAb(A5495)
Review
ABclonal: review for [KO Validated] DNMT1 Rabbit pAb(A5495)
Review
ABclonal: review for [KO Validated] DNMT1 Rabbit pAb(A5495)
Review
ABclonal: review for [KO Validated] DNMT1 Rabbit pAb(A5495)
Review
ABclonal: review for [KO Validated] DNMT1 Rabbit pAb(A5495)
Review
ABclonal: review for [KO Validated] DNMT1 Rabbit pAb(A5495)
Review
ABclonal: review for [KO Validated] DNMT1 Rabbit pAb(A5495)
Review
ABclonal: review for [KO Validated] DNMT1 Rabbit pAb(A5495)
Review
ABclonal: review for [KO Validated] DNMT1 Rabbit pAb(A5495)
Review
ABclonal: review for [KO Validated] DNMT1 Rabbit pAb(A5495)

Overview

Product name[KO Validated] DNMT1 Rabbit pAb
Catalog No.A5495
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes an enzyme that transfers methyl groups to cytosine nucleotides of genomic DNA. This protein is the major enzyme responsible for maintaining methylation patterns following DNA replication and shows a preference for hemi-methylated DNA. Methylation of DNA is an important component of mammalian epigenetic gene regulation. Aberrant methylation patterns are found in human tumors and associated with developmental abnormalities. Variation in this gene has been associated with cerebellar ataxia, deafness, and narcolepsy, and neuropathy, hereditary sensory, type IE. Alternative splicing results in multiple transcript variants.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human DNMT1 (NP_001370.1).
SequenceMPARTAPARVPTLAVPAISLPDDVRRRLKDLERDSLTEKECVKEKLNLLHEFLQTEIKNQLCDLETKLRKEELSEEGYLAKVKSLLNKDLSLENGAHAYNREVNGRLENGNQARSEARRVGMADANSPPKPLSKPRTPRRSKSDGEAKPEPSPSPRITRKSTRQTTITSHFAKGPAKRKPQEESERAKSDESIKEEDKDQDEKRRRVTSRERVARPLPAEEPERAKSGTRTEKEEERDEKEEKRLRSQTKEPTPKQKLKEEPDREARAGV
Gene ID1786
Swiss protP26358
SynonymsADCADN; AIM; CXXC9; DNMT; HSN1E; MCMT; m.HsaI; DNMT1
Calculated MW144kDa/183kDa/184kDa
Observed MW200kDa
ReactivityHuman
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IF 1:50 - 1:200
IP 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunofluorescence    Immunoprecipitation    
Positive samples293T, SW480
Cellular locationNucleus
Customer validation

(Human hepatocellular carcinoma cell line, HUVEC, SMMC7721,BEL7404, Human cells, HEK293T, BEL-7402)

IF(Leporidae, Homo sapiens)

WB(Homo sapiens, Rattus norvegicus)

ChIP(Homo sapiens)

IF(Rattus norvegicus)

IHC(Rattus norvegicus)

ABclonal:Western blot - [KO Validated] DNMT1 Polyclonal Antibody (A5495) }

Western blot - [KO Validated] DNMT1 Polyclonal Antibody (A5495)

Western blot analysis of extracts of various cell lines, using DNMT1 antibody (A5495) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Western blot - [KO Validated] DNMT1 Polyclonal Antibody (A5495) }

Western blot - [KO Validated] DNMT1 Polyclonal Antibody (A5495)

Western blot analysis of extracts from normal (control) and DNMT1 knockout (KO) 293T cells, using DNMT1 antibody (A5495) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Immunofluorescence - [KO Validated] DNMT1 Polyclonal Antibody (A5495) }

Immunofluorescence - [KO Validated] DNMT1 Polyclonal Antibody (A5495)

Immunofluorescence analysis of 293T cells using DNMT1 antibody (A5495) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunoprecipitation - [KO Validated] DNMT1 Polyclonal Antibody (A5495) }

Immunoprecipitation - [KO Validated] DNMT1 Polyclonal Antibody (A5495)

Immunoprecipitation analysis of 200ug extracts of 293T cells using 3ug DNMT1 antibody (A5495). Western blot was performed from the immunoprecipitate using DNMT1 antibody (A5495) at a dilition of 1:1000.

* For research use only. Not for therapeutic or diagnostic purposes.

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