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[KO Validated] DNAJB1 Rabbit pAb (A5504)

Publication (1) Datasheet

ABclonal:Western blot - [KO Validated] DNAJB1 Polyclonal Antibody (A5504)
ABclonal:Western blot - [KO Validated] DNAJB1 Polyclonal Antibody (A5504)
ABclonal:Immunofluorescence - [KO Validated] DNAJB1 Polyclonal Antibody (A5504)
ABclonal:Immunofluorescence - [KO Validated] DNAJB1 Polyclonal Antibody (A5504)
ABclonal:Immunofluorescence - [KO Validated] DNAJB1 Polyclonal Antibody (A5504)

Overview

Product name[KO Validated] DNAJB1 Rabbit pAb
Catalog No.A5504
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the DnaJ or Hsp40 (heat shock protein 40 kD) family of proteins. DNAJ family members are characterized by a highly conserved amino acid stretch called the 'J-domain' and function as one of the two major classes of molecular chaperones involved in a wide range of cellular events, such as protein folding and oligomeric protein complex assembly. The encoded protein is a molecular chaperone that stimulates the ATPase activity of Hsp70 heat-shock proteins in order to promote protein folding and prevent misfolded protein aggregation. Alternative splicing results in multiple transcript variants.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-340 of human DNAJB1 (NP_006136.1).
SequenceMGKDYYQTLGLARGASDEEIKRAYRRQALRYHPDKNKEPGAEEKFKEIAEAYDVLSDPRKREIFDRYGEEGLKGSGPSGGSGGGANGTSFSYTFHGDPHAMFAEFFGGRNPFDTFFGQRNGEEGMDIDDPFSGFPMGMGGFTNVNFGRSRSAQEPARKKQDPPVTHDLRVSLEEIYSGCTKKMKISHKRLNPDGKSIRNEDKILTIEVKKGWKEGTKITFPKEGDQTSNNIPADIVFVLKDKPHNIFKRDGSDVIYPARISLREALCGCTVNVPTLDGRTIPVVFKDVIRPGMRRKVPGEGLPLPKTPEKRGDLIIEFEVIFPERIPQTSRTVLEQVLPI
Gene ID3337
Swiss protP25685
SynonymsDNAJB1; HSPF1; Hdj1; Hsp40; RSPH16B; Sis1
Calculated MW27kDa/38kDa
Observed MW39kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunofluorescence    
Positive samples293T, MCF-7, BxPC-3, BT-474, SW620, K-562, Mouse thymus, Mouse spleen, Mouse thymus
Cellular locationCytoplasm, Nucleus, nucleolus
Customer validation

WB(Mus musculus)

    ABclonal:Western blot - [KO Validated] DNAJB1 Polyclonal Antibody (A5504) }

    Western blot - [KO Validated] DNAJB1 Polyclonal Antibody (A5504)

    Western blot analysis of extracts of various cell lines, using DNAJB1 antibody (A5504) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25ug per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 15s.
    ABclonal:Western blot - [KO Validated] DNAJB1 Polyclonal Antibody (A5504) }

    Western blot - [KO Validated] DNAJB1 Polyclonal Antibody (A5504)

    Western blot analysis of extracts from normal (control) and DNAJB1 knockout (KO) 293T cells, using DNAJB1 antibody (A5504) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25ug per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 1s.
    ABclonal:Immunofluorescence - [KO Validated] DNAJB1 Polyclonal Antibody (A5504) }

    Immunofluorescence - [KO Validated] DNAJB1 Polyclonal Antibody (A5504)

    Immunofluorescence analysis of U2OS cells using DNAJB1 antibody (A5504). Blue: DAPI for nuclear staining.
    ABclonal:Immunofluorescence - [KO Validated] DNAJB1 Polyclonal Antibody (A5504) }

    Immunofluorescence - [KO Validated] DNAJB1 Polyclonal Antibody (A5504)

    Immunofluorescence analysis of C6 cells using DNAJB1 antibody (A5504) at dilution of 1:100. Blue: DAPI for nuclear staining.
    ABclonal:Immunofluorescence - [KO Validated] DNAJB1 Polyclonal Antibody (A5504) }

    Immunofluorescence - [KO Validated] DNAJB1 Polyclonal Antibody (A5504)

    Immunofluorescence analysis of HeLa cells using DNAJB1 antibody (A5504) at dilution of 1:100. Blue: DAPI for nuclear staining.

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