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[KO Validated] DFFA Rabbit pAb (A1032)

Datasheet

ABclonal:Western blot - [KO Validated] DFFA Rabbit pAb (A1032)
ABclonal:Western blot - [KO Validated] DFFA Rabbit pAb (A1032)
ABclonal:Immunofluorescence - [KO Validated] DFFA Rabbit pAb (A1032)

Overview

Product name[KO Validated] DFFA Rabbit pAb
Catalog No.A1032
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-331 of human DFFA (NP_004392.1).
SequenceMEVTGDAGVPESGEIRTLKPCLLRRNYSREQHGVAASCLEDLRSKACDILAIDKSLTPVTLVLAEDGTIVDDDDYFLCLPSNTKFVALASNEKWAYNNSDGGTAWISQESFDVDETDSGAGLKWKNVARQLKEDLSSIILLSEEDLQMLVDAPCSDLAQELRQSCATVQRLQHTLQQVLDQREEVRQSKQLLQLYLQALEKEGSLLSKQEESKAAFGEEVDAVDTGISRETSSDVALASHILTALREKQAPELSLSSQDLELVTKEDPKALAVALNWDIKKTETVQEACERELALRLQQTQSLHSLRSISASKASPPGDLQNPKRARQDPT
Gene ID1676
Swiss protO00273
SynonymsDFFA; DFF-45; DFF1; ICAD
Calculated MW29kDa/36kDa
Observed MW35kDa, 45kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
IF 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunofluorescence    
Positive samplesJurkat, H460, HepG2, Mouse brain, Rat brain
Cellular locationCytoplasm
ABclonal:Western blot - [KO Validated] DFFA Rabbit pAb (A1032) }

Western blot - [KO Validated] DFFA Rabbit pAb (A1032)

Western blot analysis of extracts of various cell lines, using DFFA antibody (A1032) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 15s.
ABclonal:Western blot - [KO Validated] DFFA Rabbit pAb (A1032) }

Western blot - [KO Validated] DFFA Rabbit pAb (A1032)

Western blot analysis of extracts from normal (control) and DFFA knockout (KO) HeLa cells, using DFFA antibody (A1032) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.
ABclonal:Immunofluorescence - [KO Validated] DFFA Rabbit pAb (A1032) }

Immunofluorescence - [KO Validated] DFFA Rabbit pAb (A1032)

Immunofluorescence analysis of HeLa cells using DFFA antibody (A1032). Blue: DAPI for nuclear staining.

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