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[KO Validated] DDIT4 Rabbit pAb (A8086)

Publication (1) Datasheet

ABclonal:Western blot - [KO Validated] DDIT4 Rabbit pAb (A8086)
ABclonal:Western blot - [KO Validated] DDIT4 Rabbit pAb (A8086)

Overview

Product name[KO Validated] DDIT4 Rabbit pAb
Catalog No.A8086
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-232 of human DDIT4 (NP_061931.1).
SequenceMPSLWDRFSSSSTSSSPSSLPRTPTPDRPPRSAWGSATREEGFDRSTSLESSDCESLDSSNSGFGPEEDTAYLDGVSLPDFELLSDPEDEHLCANLMQLLQESLAQARLGSRRPARLLMPSQLVSQVGKELLRLAYSEPCGLRGALLDVCVEQGKSCHSVGQLALDPSLVPTFQLTLVLRLDSRLWPKIQGLFSSANSPFLPGFSQSLTLSTGFRVIKKKLYSSEQLLIEEC
Gene ID54541
Swiss protQ9NX09
SynonymsDDIT4; Dig2; REDD-1; REDD1
Calculated MW25kDa
Observed MW32KDa
ReactivityHuman
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    
Positive samplesA-549, DU145, U-251MG
Cellular locationCytoplasm, Mitochondrion, cytosol
Customer validation

WB(Homo sapiens,Mus musculus)

ABclonal:Western blot - [KO Validated] DDIT4 Rabbit pAb (A8086) }

Western blot - [KO Validated] DDIT4 Rabbit pAb (A8086)

Western blot analysis of extracts from normal (control) and DDIT4 knockout (KO) HeLa cells, using DDIT4 antibody (A8086) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.
ABclonal:Western blot - [KO Validated] DDIT4 Rabbit pAb (A8086) }

Western blot - [KO Validated] DDIT4 Rabbit pAb (A8086)

Western blot analysis of extracts of various cell lines, using DDIT4 antibody (A8086) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.

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