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[KO Validated] Cytochrome C Rabbit mAb (A4912)

Publications (5) Datasheet

ABclonal:Western blot - [KO Validated] Cytochrome C Rabbit mAb (A4912)

Western blot analysis of extracts of various cell lines, using Cytochrome C Rabbit mAb (A4912) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Western blot - [KO Validated] Cytochrome C Rabbit mAb (A4912)

Western blot analysis of extracts from normal (control) and Cytochrome C knockout (KO) 293T cells, using Cytochrome C antibody (A4912) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunohistochemistry - [KO Validated] Cytochrome C Rabbit mAb (A4912)

Immunohistochemistry of paraffin-embedded rat kidney using Cytochrome C Rabbit mAb (A4912) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - [KO Validated] Cytochrome C Rabbit mAb (A4912)

Immunohistochemistry of paraffin-embedded human appendix using Cytochrome C Rabbit mAb (A4912) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Overview

Product name[KO Validated] Cytochrome C Rabbit mAb
Catalog No.A4912
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a small heme protein that functions as a central component of the electron transport chain in mitochondria. The encoded protein associates with the inner membrane of the mitochondrion where it accepts electrons from cytochrome b and transfers them to the cytochrome oxidase complex. This protein is also involved in initiation of apoptosis. Mutations in this gene are associated with autosomal dominant nonsyndromic thrombocytopenia. Numerous processed pseudogenes of this gene are found throughout the human genome.[provided by RefSeq, Jul 2010]
ImmunogenA synthesized peptide derived from human Cytochrome C
SequenceEmail for sequence
Gene ID54205
Swiss protP99999
SynonymsCYC; HCS; THC4
Calculated MW15kDa
Observed MW14KDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    
Positive samplesHeLa, 293T, Mouse brain, Mouse kidney, Mouse heart, Rat brain, Rat kidney
Cellular locationMitochondrion intermembrane space
Customer validation

WB(Homo sapiens, lamprey, Mus musculus, Rattus norvegicus)

ABclonal:Western blot - [KO Validated] Cytochrome C Rabbit mAb (A4912) }

Western blot - [KO Validated] Cytochrome C Rabbit mAb (A4912)

Western blot analysis of extracts of various cell lines, using Cytochrome C Rabbit mAb (A4912) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Western blot - [KO Validated] Cytochrome C Rabbit mAb (A4912) }

Western blot - [KO Validated] Cytochrome C Rabbit mAb (A4912)

Western blot analysis of extracts from normal (control) and Cytochrome C knockout (KO) 293T cells, using Cytochrome C antibody (A4912) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Immunohistochemistry - [KO Validated] Cytochrome C Rabbit mAb (A4912) }

Immunohistochemistry - [KO Validated] Cytochrome C Rabbit mAb (A4912)

Immunohistochemistry of paraffin-embedded rat kidney using Cytochrome C Rabbit mAb (A4912) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - [KO Validated] Cytochrome C Rabbit mAb (A4912) }

Immunohistochemistry - [KO Validated] Cytochrome C Rabbit mAb (A4912)

Immunohistochemistry of paraffin-embedded human appendix using Cytochrome C Rabbit mAb (A4912) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

* For research use only. Not for therapeutic or diagnostic purposes.

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